Bub1 beta Antikörper

Produktnummer ABIN306788

Der Schaf Polyklonal anti-Bub1 beta Antikörper wird verwendet zum Nachweis von Bub1 beta in Proben von Maus. Er wurde validiert für IHC und WB.

Kurzübersicht für Bub1 beta Antikörper (ABIN306788)

Target: Bub1 beta

Reaktivität: Maus

Wirt: Schaf

Klonalität: Polyklonal

Applikation: Immunohistochemistry (IHC), Western Blotting (WB)

Produktdetails

Verwendungszweck: Sheep anti Mouse Bub1 beta-IN

Kreuzreaktivität: Maus

Produktmerkmale: Bub1, a mitotic checkpoint gene first identified in yeast, ensures proper chromosome segregation by delaying anaphase until chromosomes are properly aligned on the mitotic spindle. Mutations in the mitotic spindle assembly checkpoint genes, like yeast Bub1, cause cells to exit mitosis without proper chromosome attachment/alignment, resulting in aneuploidy. Aneuploidy is a form of genetic instability involved in cancer. Recently, a novel homologue of the murine Bub1 gene has been identified and named Bub1b. The yeast mitotic checkpoint pathway is unresolved, but the mammalian pathway remains even less clear. It is important to investigate the role of the Bub1 genes in the mammalian checkpoint as they have been implicated in carcinogenesis, namely colon carcinomas. The aneuploidy and chromosome instability characterizing some tumors is thought to be caused by mutations in Bub1.

Aufreinigung: Ammonium Sulfate Precipitation

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: X1163P antibody detects Bub1b in mitotic NIH/3T3 cells, seperated by immunoprecipitation. Westen blot analysis (1-10 μg/mL) detects a 130 kDa band. Optimal antibody concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Antigendetails

Target: Bub1 beta

Hintergrund: Mitotic checkpoint kinase MAD3L

UniProt: Q9Z1S0