BUB1B Antikörper (AA 883-1052)

Produktnummer ABIN306786

Produktdetails anti-BUB1B Antikörper

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Bindungsspezifität: AA 883-1052

Reaktivität: Maus

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser BUB1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Kreuzreaktivität: Maus

Produktmerkmale: Mitotic checkpoint kinase MAD3L,Bub1, a mitotic checkpoint gene first identified in yeast, ensures proper chromosome segregation by delaying anaphase until chromosomes are properly aligned on the mitotic spindle. Mutations in the mitotic spindle assembly checkpoint genes, like yeast Bub1, cause cells to exit mitosis without proper chromosome attachment/alignment, resulting in aneuploidy. Aneuploidy is a form of genetic instability involved in cancer. Recently, a novel homologue of the murine Bub1 gene has been identified and named Bub1b. The yeast mitotic checkpoint pathway is unresolved, but the mammalian pathway remains even less clear. It is important to investigate the role of the Bub1 genes in the mammalian checkpoint as they have been implicated in carcinogenesis, namely colon carcinomas. The aneuploidy and chromosome instability characterizing some tumors is thought to be caused by mutations in Bub1.

Aufreinigung: Ammonium Sulfate Precipitation

Immunogen: Recombinant protein corresponding to amino acids 883-1052 of the mouse Bub1b protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Antibody detects Bub1b in mitotic cells seperated from NIH/3T3 cells by IP/Westen blot (1-10 μg/mL) analysis. Detects a 130 kDa band. Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu BUB1B

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Andere Bezeichnung: Bub1 beta (BUB1B Produkte)

UniProt: Q9Z1S0