PIG3 (p53 Inducible Gene 3) Antikörper

Produktnummer ABIN306783

Der Maus Monoklonal anti-PIG3 (p53 Inducible Gene 3) Antikörper (Klon 10A2) (ABIN306783) detektiert spezifisch PIG3 (p53 Inducible Gene 3) in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für PIG3 (p53 Inducible Gene 3) Antikörper (ABIN306783)

Target: PIG3 (p53 Inducible Gene 3)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB)

Klon: 10A2

Produktdetails

Verwendungszweck: Mouse anti Human PIG3 (p53 inducible gene 3)

Kreuzreaktivität: Human

Produktmerkmale: p53 inducible gene-3 (PIG-3) may be a long-lived reporter, which may be useful for detecting transient activation of p53. The p53 tumor suppressor is the most commonly mutated gene in human cancers. The p53 protein, which is stabilized in response to different biological checkpoints, is activated by DNA damage, hypoxia, viral infection, or oncogene activation resulting in efects such as cell cycle arrest, apoptosis, senescence, differentiation, and antiangiogenesis. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others. The p53-inducible gene 3 (PIG3), was recently identified in a screen for genes induced by p53 before the onset of apoptosis. PIG3 shares significant homology with the oxidoreductases from several species. PIG3 protein is localized to the cytoplasm and induced in primary, non-transformed, and transformed cell cultures after exposure to genotoxic agents. The induction of PIG3 by p53 occurrs with delayed kinetics as compared with other p53 downstream targets, such as p21 and MDM2. PIG3 levels are increased during p53-mediated growth arrest. Elevated levels of PIG3 are maintained in cells that resume cycling in the absence of ectopic p53 expression.

Aufreinigung: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human PIG-3 protein.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Antibody can be used for Western blotting (5-10 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Antigendetails

Target: PIG3 (p53 Inducible Gene 3)

Hintergrund: Putative quinone oxidoreductase, Tumor protein p53-inducible protein 3, p53-induced gene 3 protein, TP53I3, PIG3