S1PR1 Antikörper (C-Term)

Produktnummer ABIN306730

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch S1PR1 in WB. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für S1PR1 Antikörper (C-Term) (ABIN306730)

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser S1PR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-S1PR1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Rabbit anti Human Sphingosine 1 Phosphate Receptor 1 (EDG-1) CT

Kreuzreaktivität: Human, Maus, Ratte

Produktmerkmale: EDG-1 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-1 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-1 is expressed in cardiovascular, leukocyte- containing and other tissues.

Aufreinigung: Ammonium Sulfate Precipitation

Immunogen: Synthetic peptide derived from the C-terminal of the EDG-1 receptor

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Detects EDG1 receptors at concentration of 5-10 μg/mL by Western blot using a human EDG1 receptor transfected cell line. Detects an approximately 44 kDa band in RH7777 cells transfected with full length human EDG1. Due to low expression of EDG receptors, we recommend use of Pierce Femto Signal substrate for western blot development. Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu S1PR1

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Andere Bezeichnung: Sphingosine 1 Phosphate Receptor 1 (EDG-1)

Hintergrund: Endothelial cell differentiation gene 1 C-terminal

UniProt: P21453

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2