NAPA Antikörper

Produktnummer ABIN306712

Produktdetails anti-NAPA Antikörper

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NAPA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Spezifität: Human alpha -SNAP

Kreuzreaktivität: Rind (Kuh), Human, Ratte

Produktmerkmale: alpha/beta SNAP, mouse anti alpha soluble NSF attachment protein,SNAPA,SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. Beta-SNAP (or SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas alpha-SNAP is expressed in all mammalian tissues, beta-SNAP is expressed only in brain.

Aufreinigung: The antibody was purified to >95% homogeneity by standard chromatographic techniques

Immunogen: Balb/C mice were immunized with recombinant human alpha -SNAP. The resultant hybridomas were selected for reactivity with the immunogen by ELISA and with the antigen by Western blot.,Monoclonal antibody 15D4 obtained immunization of Balb/C mice with recombinant human alpha -SNAP

Klon: 15D4

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal concentration should be evaluated by serial dilutions. Clone 4E4 anti-?SNAP specifically recognizes ?-SNAP as a single band of ~36 kDa on western blot of rat kidney, rat brain and MDBK cells and does not recognize ?-SNAP Application: A dilution of 0.5-5 μg/mL is suggested for western blot and 2-10 μg/mL for immunoprecipitation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50 % glycerol. No preservatives have been included in the antibody solution.,100 μg monoclonal antibody in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50 % glycerol. No preservatives have been added.

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu NAPA

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Andere Bezeichnung: alpha SNAP (NAPA Produkte)

Synonyme: SNAPA antikoerper, wu:fa26c04 antikoerper, zgc:63906 antikoerper, napb antikoerper, 1500039N14Rik antikoerper, AW209189 antikoerper, RA81 antikoerper, SNARE antikoerper, a-SNAP antikoerper, hyh antikoerper, alpha-SNAP antikoerper, Alpha-SNAP antikoerper, NSF attachment protein alpha antikoerper, N-ethylmaleimide-sensitive factor attachment protein, alpha b antikoerper, NSF attachment protein alpha L homeolog antikoerper, N-ethylmaleimide-sensitive factor attachment protein, alpha antikoerper, N-ethylmaleimide sensitive fusion protein attachment protein alpha antikoerper, soluble NSF attachment protein antikoerper, NAPA antikoerper, napab antikoerper, napa.L antikoerper, napa antikoerper, EHI_000300 antikoerper, Napa antikoerper, LOC102577508 antikoerper

Pathways: Synaptic Vesicle Exocytosis, Asymmetric Protein Localization