NAPA Antikörper

Produktnummer ABIN306712

Dieser Maus Monoklonal Antikörper erkennt spezifisch NAPA in WB, IP und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für NAPA Antikörper (ABIN306712)

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NAPA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Klon: 15D4

Produktdetails anti-NAPA Antikörper

Spezifität: Human alpha -SNAP

Kreuzreaktivität: Rind (Kuh), Human, Ratte

Produktmerkmale: alpha/beta SNAP, mouse anti alpha soluble NSF attachment protein,SNAPA,SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. Beta-SNAP (or SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas alpha-SNAP is expressed in all mammalian tissues, beta-SNAP is expressed only in brain.

Aufreinigung: The antibody was purified to >95% homogeneity by standard chromatographic techniques

Immunogen: Balb/C mice were immunized with recombinant human alpha -SNAP. The resultant hybridomas were selected for reactivity with the immunogen by ELISA and with the antigen by Western blot.,Monoclonal antibody 15D4 obtained immunization of Balb/C mice with recombinant human alpha -SNAP

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal concentration should be evaluated by serial dilutions. Clone 4E4 anti-?SNAP specifically recognizes ?-SNAP as a single band of ~36 kDa on western blot of rat kidney, rat brain and MDBK cells and does not recognize ?-SNAP Application: A dilution of 0.5-5 μg/mL is suggested for western blot and 2-10 μg/mL for immunoprecipitation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50 % glycerol. No preservatives have been included in the antibody solution.,100 μg monoclonal antibody in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50 % glycerol. No preservatives have been added.

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu NAPA

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Andere Bezeichnung: alpha SNAP

Pathways: Synaptic Vesicle Exocytosis, Asymmetric Protein Localization