CAPN1 Antikörper

Produktnummer ABIN306692

Der Maus Monoklonal Anti-CAPN1 Antikörper wurde für WB, IP und EIA validiert. Er ist geeignet, CAPN1 in Proben von Human zu detektieren.

Kurzübersicht für CAPN1 Antikörper (ABIN306692)

Target: CAPN1 (Calpain 1 (CAPN1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CAPN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Klon: 28F3

Produktdetails anti-CAPN1 Antikörper

Kreuzreaktivität: Rind (Kuh), Human

Produktmerkmale: Mouse anti calpain I, II small subunit, p30,The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and μ-calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar ( mM) levels of calcium, μ-calpain is active at micromolar (μM) concentrations of Ca ++. In addition to the ubiquitously expressed m- and μcalpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.

Aufreinigung: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with human calpain with mouse myeloma.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Use at 0.5-1 μg/mL for ELISA and western blot, 1-2 μg/mL for immunoprecipitation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Provided as a concentration of 1 mg/mL in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50 % glycerol, 3 mM sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu CAPN1

Target: CAPN1 (Calpain 1 (CAPN1))

Andere Bezeichnung: Calpain I

UniProt: P17655