VAV2 Antikörper

Produktnummer ABIN306672

Der Schaf Polyklonal anti-VAV2 Antikörper (ABIN306672) detektiert spezifisch VAV2 in WB und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für VAV2 Antikörper (ABIN306672)

Target: VAV2 (Vav 2 Guanine Nucleotide Exchange Factor (VAV2))

Reaktivität: Human

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser VAV2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-VAV2 Antikörper

Verwendungszweck: Sheep anti Human Vav-2 SHD

Kreuzreaktivität: Maus

Produktmerkmale: The immunogen used for the anti-human Vav-2 SHD region was a his tagged fusion protein Vav-2 corresponding to amino acid sequence 578 - 878 and having a molecular weight of 100kD. Vav-2 has a molecular weight of 100 kD and shares over a 50 % homology at the amino acid and nucleic acid level with Vav. Vav-2 is a ubiquitously expressed structural homolog of the Vav protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl- homology ((DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type vav-2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a Vav-2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.

Aufreinigung: Ammonium Sulfate Precipitation

Immunogen: A his tagged fusion protein Vav-2 corresponding to amino acid sequence 578 - 878 and having a molecular weight of 100kD

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Applications: Western Blot 1:5,000. Immunoprecipitation -Empirical

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu VAV2

Target: VAV2 (Vav 2 Guanine Nucleotide Exchange Factor (VAV2))

Andere Bezeichnung: Vav-2 SHD

Hintergrund: VAV-2, Guanine nucleotide exchange factor VAV-2, Src homology 2 and 3 (SH2 & SH3) domains

UniProt: P52735

Pathways: Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung