P110 (Mitochondrial Membrane Protein) Antikörper

Produktnummer ABIN306552

Der Maus Monoklonal anti-P110 (Mitochondrial Membrane Protein) Antikörper (Klon 2G2) (ABIN306552) detektiert spezifisch P110 (Mitochondrial Membrane Protein) in IF und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Affe.

Kurzübersicht für P110 (Mitochondrial Membrane Protein) Antikörper (ABIN306552)

Target: P110 (Mitochondrial Membrane Protein)

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunofluorescence (IF), Western Blotting (WB)

Klon: 2G2

Produktdetails

Verwendungszweck: Mouse anti Human P110 (mitochondrial membrane protein)

Kreuzreaktivität: Human

Produktmerkmale: P110 is a human mitochondrial protein with a molecular weight of 110 kDa. Close examination of the staining pattern in HeLa and Fanconi's Anemia cells reveal differences in the morphology and organization of mitochondria in these two cell types. The epitope targeted may serve as a valuable marker in the investigation of relationships between mitochondria and other cellular structures in human cells. The mouse monoclonal P110 antibody labels mitochondria in human cells, as assessed by double staining with either Rhodamine 123 or a polyclonal antibody to mitochondrial matrix HSP-60 proteins. The P110 antigen has an approximate isoelectric point of 6.5 that copartitions with HSP-60 proteins during isolation of mitochondria from HeLa cells. The P110 staining pattern in HeLa and Fanconi's anaemia cells reveals differences in the morphology and organization of mitochondria in these two cell types. In HeLa cells, mitochondria appear as individual tubular compartments of variable length and are closely associated with vimentin filaments, particularly at the periphery of the nucleus. In Fanconi's anaemia cells, mitochondria have a filamentous shape and form an interconnected cytoplasmic reticulum running in parallel with both vimentin filaments and microtubules. After stabilization with aldehyde- or alcohol-based fixation protocols that optimize the preservation of cytoskeletal components, the epitope targeted by the 2G2 antibody may serve as a valuable marker in the investigation of relationships between mitochondria and other cellular structures in human cells.

Aufreinigung: Protein A/G Chromatography

Immunogen: Skeletal fracrtion of HeLa-S3 cells

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Application: Western Blot at 2-10 μg/mL. Immunofluorescence staining.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Antigendetails

Target: P110 (Mitochondrial Membrane Protein)

Hintergrund: 2G2, mitochondrial membrane protein, p110

UniProt: Q9KGY0