JUP Antikörper (AA 732-745)

Produktnummer ABIN305904

Der Ziege Polyklonal anti-JUP Antikörper (ABIN305904) detektiert spezifisch JUP in WB, IHC, ELISA und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Hund, Kaninchen, Affe und Hamster.

Kurzübersicht für JUP Antikörper (AA 732-745) (ABIN305904)

Target: JUP (Junction Plakoglobin (JUP))

Reaktivität: Human, Maus, Hund, Kaninchen, Affe, Hamster

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser JUP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-JUP Antikörper

Bindungsspezifität: AA 732-745

Spezifität: Human JUP / Gamma Catenin. This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Dog, Hamster, Panda, Rabbit (100%) Rat, Bat, Bovine, Pig, Opossum (93%) Elephant (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-GLRPPYPTADHMLA from the C-terminus of human JUP / Gamma Catenin (NP_002221.1, NP_068831.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Dog, Hamster, Panda, Rabbit (100%), Rat, Bat, Bovine, Pig, Opossum (93%), Elephant (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu JUP

Target: JUP (Junction Plakoglobin (JUP))

Andere Bezeichnung: JUP/CTNNG/Junction Plakoglobin

Hintergrund: Name/Gene ID: JUP
Family: Catenin

Synonyms: JUP, Desmoplakin-3, CTNNG, DPIII, Gamma catenin, Junction plakoglobin, PKGB, ARVD12, Catenin gamma, Desmoplakin III, DP3, PDGB

Gen-ID: 3728

NCBI Accession: NP_002221, NP_068831

UniProt: P14923

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location