Occludin Antikörper (C-Term)
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- Target Alle Occludin (OCLN) Antikörper anzeigen
- Occludin (OCLN)
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Bindungsspezifität
- C-Term
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Reaktivität
- Human, Maus, Ratte, Hund
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Occludin Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Recognizes human occludin at ~65kD. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, human Caco-2 and rat fibroblasts transfected with a plasmid encoding human occludin. Species cross-reactivity has been confirmed in Western blot only.
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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A GST fusion protein consisting of the C-terminal 150 amino acids of human occludin fused to GST.
Type of Immunogen: Fusion protein - Isotyp
- IgG
- Top Product
- Discover our top product OCLN Primärantikörper
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- Applikationshinweise
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Approved: ELISA (0.1 - 1 μg/mL), IF, IHC, IHC-P (2.5 μg/mL), IP, WB (1 - 3 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS, pH 7.4, 0.09 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeat freeze-thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- Occludin (OCLN)
- Andere Bezeichnung
- OCLN / Occludin (OCLN Produkte)
- Synonyme
- AI503564 antikoerper, Ocl antikoerper, ocln antikoerper, oclnb antikoerper, tpmt antikoerper, OCLN antikoerper, wu:fd23h10 antikoerper, wu:fi13c01 antikoerper, zgc:113992 antikoerper, zgc:56359 antikoerper, BLCPMG antikoerper, occludin antikoerper, occludin S homeolog antikoerper, occludin a antikoerper, Ocln antikoerper, OCLN antikoerper, ocln.S antikoerper, ocln antikoerper, oclna antikoerper
- Hintergrund
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Name/Gene ID: OCLN
Synonyms: OCLN, Occludin, BLCPMG - Gen-ID
- 100506658
- UniProt
- Q16625
- Pathways
- Cell-Cell Junction Organization, Hepatitis C
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