Claudin 12 Antikörper (N-Term)

Produktnummer ABIN305786

Der Kaninchen Polyklonal Anti-Claudin 12-Antikörper wurde für WB, IHC, IHC (p) und IF validiert. Er ist geeignet, Claudin 12 in Proben von Human und Maus zu detektieren.

Kurzübersicht für Claudin 12 Antikörper (N-Term) (ABIN305786)

Target: Claudin 12 (CLDN12)

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Claudin 12 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-Claudin 12 Antikörper

Bindungsspezifität: N-Term

Marke: IHC-plus™

Spezifität: Recognizes mouse Claudin 12 protein at ~27kD in Western blots. Cross-reactivity has been confirmed on mouse lung, colon and kidney homogenates. Recognizes human protein in IHC-P.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the N-terminal region of mouse and human Claudin 12.

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IF (5 μg/mL), IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunofluorescence: 5 μg/mL, performed using ethanol-fixed mouse colon. Positive control: Mouse lung, colon, and kidney homogenates.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Aliquot to Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Claudin 12

Target: Claudin 12 (CLDN12)

Andere Bezeichnung: CLDN12 / Claudin 12

Hintergrund: Name/Gene ID: CLDN12
Family: Claudin

Synonyms: CLDN12, Claudin 12, Claudin-12

Gen-ID: 9069

UniProt: P56749

Pathways: Cell-Cell Junction Organization