ABCB11 Antikörper (AA 705-718)

Produktnummer ABIN305775

Dieses Anti-ABCB11-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ABCB11 in WB, IHC, ICC und IHC (p). Geeignet für Human.

Kurzübersicht für ABCB11 Antikörper (AA 705-718) (ABIN305775)

Target: ABCB11 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 11 (ABCB11))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ABCB11 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ABCB11 Antikörper

Bindungsspezifität: AA 705-718

Marke: IHC-plus™

Spezifität: Recognizes human BSEP. Does not cross-react with human P-glycoprotein.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda (100%) Marmoset, Dog (93%).

Aufreinigung: Protein G purified

Immunogen: Synthetic peptide corresponding to aa 705-718 (Y705LVHEPPLAVVDHK718) of human BSEP (bile salt export pump). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda (100%), Marmoset, Dog (93%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.Western Blot: 1:50-1:200Immunocytochemistry: Cytospin preparationsOptimal dilutions to be determined by the researcher.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS  pH 7.2, 0.7 % BSA, 100nM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu ABCB11

Target: ABCB11 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 11 (ABCB11))

Andere Bezeichnung: ABCB11 / BSEP

Hintergrund: Name/Gene ID: ABCB11
Subfamily: ATP-binding cassette - ABCB/MDR
Family: Transporter

Synonyms: ABCB11, ABC16, Bile salt export pump, BSEP, PGY4, Sister p-glycoprotein, BRIC2, PFIC-2, PFIC2, SPGP

Gen-ID: 8647

UniProt: O95342