DNMT1 Antikörper (AA 637-650)

Produktnummer ABIN305735

Der Maus Monoklonal Anti-DNMT1 Antikörper wurde für WB, IHC, IP, ChIP und IHC (p) validiert. Er ist geeignet, DNMT1 in Proben von Human, Maus, Affe, Pferd, Kaninchen und Fledermaus zu detektieren.

Kurzübersicht für DNMT1 Antikörper (AA 637-650) (ABIN305735)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reaktivität: Human, Maus, Affe, Pferd, Kaninchen, Fledermaus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DNMT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 60B1220-1

Produktdetails anti-DNMT1 Antikörper

Bindungsspezifität: AA 637-650

Spezifität: A synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370). It will cross react with mouse Dnmt1.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Bat, Horse, Rabbit (100%) Sheep, Hamster, Elephant, Dog, Bovine, Pig (93%).

Aufreinigung: Protein G purified

Immunogen: A synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370). It will cross react with mouse Dnmt1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Bat, Horse, Rabbit (100%), Sheep, Hamster, Elephant, Dog, Bovine, Pig (93%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ChrIP, IHC, IHC-P (5 μg/mL), IP, WB (2 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Andere Bezeichnung: DNMT / DNMT1

Hintergrund: Name/Gene ID: DNMT1
Family: C5-methyltransferase

Synonyms: DNMT1, AIM, CXXC finger protein 9, CXXC9, DNA methyltransferase 1, DNMT, DNA methyltransferase HsaI, DNA MTase HsaI, HSN1E, M.HsaI, MCMT

Gen-ID: 1786

NCBI Accession: NP_001370

UniProt: P26358

Pathways: SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion