His Tag Antikörper (FITC)

Produktnummer ABIN304882

Produktdetails anti-His Tag Antikörper (FITC)

Target: His Tag

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist konjugiert mit FITC

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Sequenz: HHHHHH

Spezifität: Recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian , , and cell lines. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. Is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.

Aufreinigung: Protein G purified

Immunogen: PAX6 transcription factor linked to histidine tag.

Type of Immunogen: Fusion protein

Klon: AD1-1-10

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: Flo (1:10), IHC

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 1 % BSA.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Product is photosensitive and should be protected from light. Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.

Details zu His Tag

Target: His Tag

Abstract: His Tag Produkte

Substanzklasse: Tag