SKP2 Antikörper (N-Term)

Produktnummer ABIN3044574

Dieses Anti-SKP2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SKP2 in WB und IHC. Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 7+ Publikationen zitiert.

Kurzübersicht für SKP2 Antikörper (N-Term) (ABIN3044574)

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SKP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-SKP2 Antikörper

Bindungsspezifität: AA 2-20, N-Term

Verwendungszweck: Anti-SKP2 Antibody Picoband®

Sequenz: HRKHLQEIPD LSSNVATSF

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-SKP2 Antibody (ABIN3044574). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SKP2, different from the related rat and mouse sequences by three amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Kitagawa, M., Lee, S. H., McCormick, F. : Skp2 suppresses p53-dependent apoptosis by inhibiting p300. Molec. Cell 29: 217-231, 2008. 2. Latres, E., Chiarle, R., Schulman, B. A., Pavletich, N. P., Pellicer, A., Inghirami, G., Pagano, M. : Role of the F-box protein Skp2 in lymphomagenesis. Proc. Nat. Acad. Sci. 98: 2515-2520, 2001.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-SKP2 Antikörper (ABIN3044574)

Chen, Xie, Zhao, Wang, Bai, Yin, Jiang, Xie, Jia, Huang et al.: "Ampelopsin induces apoptosis by regulating multiple c?Myc/S?phase kinase?associated protein 2/F?box and WD repeat?containing protein 7/histone deacetylase 2 pathways in human lung adenocarcinoma ..." in: Molecular medicine reports, Vol. 11, Issue 1, pp. 105-12, (2014) (PubMed).

Xu, Zhu, Han, You, Liu, Ye, Zhang, Wang, Yao: "The oncoprotein hepatitis B X-interacting protein promotes the migration of ovarian cancer cells through the upregulation of S-phase kinase-associated protein 2 by Sp1." in: International journal of oncology, Vol. 45, Issue 1, pp. 255-63, (2014) (PubMed).

Chen, Bai, Zhong, Xie, Long, Yang, Wu, Jia, Wang: "Wogonin has multiple anti-cancer effects by regulating c-Myc/SKP2/Fbw7? and HDAC1/HDAC2 pathways and inducing apoptosis in human lung adenocarcinoma cell line A549." in: PLoS ONE, Vol. 8, Issue 11, pp. e79201, (2013) (PubMed).

Qiu, Zhang, Lv, Zhang, Zhu, Wang, Yao: "Cdh1 inhibits reactive astrocyte proliferation after oxygen-glucose deprivation and reperfusion." in: Neurochemistry international, Vol. 63, Issue 2, pp. 87-92, (2013) (PubMed).

Xu, You, Liu, Shen, Yao, Ye, Zhang: "The oncoprotein HBXIP up-regulates Skp2 via activating transcription factor E2F1 to promote proliferation of breast cancer cells." in: Cancer letters, Vol. 333, Issue 1, pp. 124-32, (2013) (PubMed).

Su, Wang, Qi, Zhao, Li, Cui: "Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts." in: Molecular vision, Vol. 17, pp. 247-56, (2011) (PubMed).

Su, Wang, Yan, Teng, Cui: "Inhibition of proliferation of rabbit lens epithelial cells by S-phase kinase-interacting protein 2 targeting small interfering RNA." in: Molecular vision, Vol. 16, pp. 907-15, (2010) (PubMed).

Details zu SKP2

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Andere Bezeichnung: SKP2

Hintergrund:

Synonyms: S-phase kinase-associated protein 2,Cyclin-A/CDK2-associated protein p45,F-box protein Skp2,F-box/LRR-repeat protein 1,p45skp2,SKP2,FBXL1,

Tissue Specificity: Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland. .

Background: The F box protein Skp2 (S-phase kinase-associated protein 2) is oncogenic, and its frequent amplification and overexpression correlate with the grade of malignancy of certain tumors. Skp2 controls p300-p53 signaling pathways in cancer cells, making it a potential molecular target for cancer therapy. This gene positively regulates the G (1)-S transition by controlling the stability of several G (1) regulators, such as the cell cycle inhibitor p27. This study provides evidence of a role for an F-box protein in oncogenesis and establishes SKP2 as a protooncogene causally involved in the pathogenesis of lymphomas.

Sequence Similarities: Contains 1 F-box domain.

Molekulargewicht: 25 kDa

UniProt: Q13309

Pathways: Mitotic G1-G1/S Phases