Ki-67 Antikörper (AA 2860-3256)

Produktnummer ABIN3044570

Produktdetails anti-Ki-67 Antikörper

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Bindungsspezifität: AA 2860-3256

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ki-67 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Verwendungszweck: Rabbit IgG polyclonal antibody for Antigen KI-67(MKI67) detection. Tested with WB, IHC-P, ICC in Human.

Marke: Picoband™

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Antigen KI-67(MKI67) detection. Tested with WB, IHC-P, ICC in Human.
Gene Name: antigen identified by monoclonal antibody Ki-67
Protein Name: Antigen KI-67

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Ki67 recombinant protein (Position: K2860-I3256).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Ki67 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-Ki-67 Antikörper (ABIN3044570)

Lu, Wang, Zhang, Liu, Li, Zhang, Ye, Zhao, Zhao, Yan, Yang, Zhang, Li, Ren: "HER2-siRNA delivered by EGFR-specific single chain antibody inhibits NSCLC cell proliferation and tumor growth." in: Oncotarget, Vol. 7, Issue 17, pp. 23594-607, (2018) (PubMed).

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Pan, Tian, Zhang, Zhang, Shi, Liang, Wu, Li, You, Yang, Mao, Qian, Xu: "YAP signaling in gastric cancer-derived mesenchymal stem cells is critical for its promoting role in cancer progression." in: International journal of oncology, Vol. 51, Issue 4, pp. 1055-1066, (2018) (PubMed).

Shi, Lv, Nie, Lu, Liu, Tian, Li, Zhang, Ren, Zhang, Kang: "Human nail stem cells are retained but hypofunctional during aging." in: Journal of molecular histology, Vol. 49, Issue 3, pp. 303-316, (2018) (PubMed).

Ling, Guan, You, Wang, Hu, Zhang, Wang, Chen, Xu, Chen: "Aloperine executes antitumor effects through the induction of apoptosis and cell cycle arrest in prostate cancer in vitro and in vivo." in: OncoTargets and therapy, Vol. 11, pp. 2735-2743, (2018) (PubMed).

Sun, Zhang, Wang, Shang, Zhou, Wang, Duan, Ruan, Gao, Dai, Qu, Liu, Ding, Wang, Wang, Dou: "MicroRNA-150 suppresses cell proliferation and metastasis in hepatocellular carcinoma by inhibiting the GAB1-ERK axis." in: Oncotarget, Vol. 7, Issue 10, pp. 11595-608, (2017) (PubMed).

Zhou, Wang, Xu, Wan, Qu, Fan, Chen, Sun, Liu: "Nek7 is overexpressed in hepatocellular carcinoma and promotes hepatocellular carcinoma cell proliferation in vitro and in vivo." in: Oncotarget, Vol. 7, Issue 14, pp. 18620-30, (2017) (PubMed).

Jiang, He, Wang, Zhang, Zhang, Yan, Wang, Che, Ke, Chen, Tong, Zhang, Wang, Li, Wan: "Mutant p53 induces EZH2 expression and promotes epithelial-mesenchymal transition by disrupting p68-Drosha complex assembly and attenuating miR-26a processing." in: Oncotarget, Vol. 6, Issue 42, pp. 44660-74, (2016) (PubMed).

Gao, Shen, He, Xiao: "GNRs@SiO₂-FA in combination with radiotherapy induces the apoptosis of HepG2 cells by modulating the expression of apoptosis-related proteins." in: International journal of molecular medicine, Vol. 36, Issue 5, pp. 1282-90, (2016) (PubMed).

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Alsaegh, Miyashita, Zhu: "Expression of human papillomavirus is correlated with Ki-67 and COX-2 expressions in keratocystic odontogenic tumor." in: Pathology oncology research : POR, Vol. 21, Issue 1, pp. 65-71, (2015) (PubMed).

Li, Wang, Wu, Yang, Zhao, Chen, Xie, Li, Wei, Huang, Zhou, Du: "miR-494-3p Regulates Cellular Proliferation, Invasion, Migration, and Apoptosis by PTEN/AKT Signaling in Human Glioblastoma Cells." in: Cellular and molecular neurobiology, Vol. 35, Issue 5, pp. 679-87, (2015) (PubMed).

Liu, Wang, Wang: "Controllable organization and high throughput production of recoverable 3D tumors using pneumatic microfluidics." in: Lab on a chip, Vol. 15, Issue 4, pp. 1195-204, (2015) (PubMed).

Huang, Ma, Xu, Li, Li, Yue, Li, Guo, Qi: "Wnt2 promotes non-small cell lung cancer progression by activating WNT/?-catenin pathway." in: American journal of cancer research, Vol. 5, Issue 3, pp. 1032-46, (2015) (PubMed).

Cheng, Wang, Li, Wu, Han, Li, Chen, Xie, Huang, Du, Zhou: "MicroRNA-184 inhibits cell proliferation and invasion, and specifically targets TNFAIP2 in Glioma." in: Journal of experimental & clinical cancer research : CR, Vol. 34, pp. 27, (2015) (PubMed).

Xie, Zhu, Yang, Huang, Wu, Wang, Luo, Wang, Shao: "Solamargine triggers hepatoma cell death through apoptosis." in: Oncology letters, Vol. 10, Issue 1, pp. 168-174, (2015) (PubMed).

Yu, Chen, Su, Huo, Lin, Liao: "Factors influencing improvement of visual field after trans-sphenoidal resection of pituitary macroadenomas: a retrospective cohort study." in: International journal of ophthalmology, Vol. 8, Issue 6, pp. 1224-8, (2015) (PubMed).

Shan, Li, Newton, Zhao, Li, Guo: "A novel protein extracted from foxtail millet bran displays anti-carcinogenic effects in human colon cancer cells." in: Toxicology letters, Vol. 227, Issue 2, pp. 129-38, (2014) (PubMed).

Details zu Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Andere Bezeichnung: MKI67 (MKI67 Produkte)

Hintergrund: Ki-67(Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

Synonyms: Antigen identified by monoclonal antibody Ki 67 antibody|Antigen KI-67 antibody|Antigen KI67 antibody|KI67_HUMAN antibody|KIA antibody|MKI67 antibody|Proliferation related Ki 67 antigen antibody|RP11-380J17.2 antibody

Gen-ID: 4288

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process