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ZP2 Antikörper (N-Term)

ZP2 Reaktivität: Human WB, IHC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3044563
  • Target Alle ZP2 Antikörper anzeigen
    ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))
    Bindungsspezifität
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 511-544, N-Term
    Reaktivität
    • 16
    • 8
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 14
    • 6
    • 1
    Kaninchen
    Klonalität
    • 15
    • 6
    Polyklonal
    Konjugat
    • 18
    • 1
    • 1
    • 1
    Dieser ZP2 Antikörper ist unkonjugiert
    Applikation
    • 15
    • 10
    • 7
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC)
    Verwendungszweck
    Anti-ZP2 Antibody Picoband®
    Sequenz
    ENEYPLVRFL RQPIYMEVRV LNRDDPNIKL VLDD
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins.
    Produktmerkmale
    Anti-ZP2 Antibody Picoband® (ABIN3044563). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence at the N-terminus of human ZP2, different from the related mouse sequence by six amino acids, and from the related rat sequence by four amino acids.
    Isotyp
    IgG
    Top Product
    Discover our top product ZP2 Primärantikörper
  • Applikationshinweise
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
    Western blot, 0.1-0.5 μg/mL
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
    1. "Entrez Gene: ZP2 zona pellucida glycoprotein 2 (sperm receptor)". 2. Burkart AD, Xiong B, Baibakov B, Jiménez-Movilla M, Dean J. "Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy.". J Cell Biol 197: 37-44. 3. Liang LF, Dean J (Apr 1993). "Conservation of mammalian secondary sperm receptor genes enables the promoter of the human gene to function in mouse oocytes". Dev Biol 156 (2): 399-408.
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target
    ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))
    Andere Bezeichnung
    ZP2 (ZP2 Produkte)
    Hintergrund

    Synonyms: Zona pellucida sperm-binding protein 2,Zona pellucida glycoprotein 2,Zp-2,Zona pellucida protein A,Processed zona pellucida sperm-binding protein 2,ZP2,ZPA,

    Tissue Specificity: Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In the spleen, mainly expressed in the white pulp germinal centers (at protein level). Up-regulated in thymic lymphomas. .

    Background: Zona pellucida sperm-binding protein 2 is a protein that in humans is encoded by the ZP2 gene. The sperm-binding domain on the ZP2 protein is necessary in both humans and mice for oocyte-sperm recognition and penetration of the zona pellucida. It is also responsible for the primary block to polyspermy in mammals. The oocyte has cortical granules peripherally located under the cortex that contain a proteolytic protein called ovastacin. After the sperm binds to ZP2, the cortical granules are exocytosed releasing ovastacin into the perivitelline space. Ovastacin cleaves ZP2 at the N terminus, preventing more sperm from binding and penetrating the oocyte, thus hardening the zona pellucida. Ovastacin is only found in oocytes, and is part of the astacin family of metalloendoproteases. Female mice engineered without ovastacin showed that ZP2 was not cleaved after fertilization.

    Molekulargewicht
    82 kDa
    Gen-ID
    7783
    UniProt
    Q05996
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