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ZP2 Antikörper (N-Term)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ZP2 in WB und IHC. Er zeigt eine Reaktivität gegenüber Human.
Produktnummer ABIN3044563

Kurzübersicht für ZP2 Antikörper (N-Term) (ABIN3044563)

Target

Alle ZP2 Antikörper anzeigen
ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))

Reaktivität

  • 16
  • 8
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 14
  • 6
  • 1
Kaninchen

Klonalität

  • 15
  • 6
Polyklonal

Konjugat

  • 18
  • 1
  • 1
  • 1
Dieser ZP2 Antikörper ist unkonjugiert

Applikation

  • 15
  • 10
  • 7
  • 5
  • 3
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC)
  • Bindungsspezifität

    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 511-544, N-Term

    Verwendungszweck

    Anti-ZP2 Antibody Picoband®

    Sequenz

    ENEYPLVRFL RQPIYMEVRV LNRDDPNIKL VLDD

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins.

    Produktmerkmale

    Anti-ZP2 Antibody Picoband® (ABIN3044563). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the N-terminus of human ZP2, different from the related mouse sequence by six amino acids, and from the related rat sequence by four amino acids.

    Isotyp

    IgG
  • Applikationshinweise

    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
    Western blot, 0.1-0.5 μg/mL
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
    1. "Entrez Gene: ZP2 zona pellucida glycoprotein 2 (sperm receptor)". 2. Burkart AD, Xiong B, Baibakov B, Jiménez-Movilla M, Dean J. "Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy.". J Cell Biol 197: 37-44. 3. Liang LF, Dean J (Apr 1993). "Conservation of mammalian secondary sperm receptor genes enables the promoter of the human gene to function in mouse oocytes". Dev Biol 156 (2): 399-408.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))

    Andere Bezeichnung

    ZP2

    Hintergrund

    Synonyms: Zona pellucida sperm-binding protein 2,Zona pellucida glycoprotein 2,Zp-2,Zona pellucida protein A,Processed zona pellucida sperm-binding protein 2,ZP2,ZPA,

    Tissue Specificity: Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In the spleen, mainly expressed in the white pulp germinal centers (at protein level). Up-regulated in thymic lymphomas. .

    Background: Zona pellucida sperm-binding protein 2 is a protein that in humans is encoded by the ZP2 gene. The sperm-binding domain on the ZP2 protein is necessary in both humans and mice for oocyte-sperm recognition and penetration of the zona pellucida. It is also responsible for the primary block to polyspermy in mammals. The oocyte has cortical granules peripherally located under the cortex that contain a proteolytic protein called ovastacin. After the sperm binds to ZP2, the cortical granules are exocytosed releasing ovastacin into the perivitelline space. Ovastacin cleaves ZP2 at the N terminus, preventing more sperm from binding and penetrating the oocyte, thus hardening the zona pellucida. Ovastacin is only found in oocytes, and is part of the astacin family of metalloendoproteases. Female mice engineered without ovastacin showed that ZP2 was not cleaved after fertilization.

    Molekulargewicht

    82 kDa

    Gen-ID

    7783

    UniProt

    Q05996
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