Fatty Acid Synthase Antikörper (AA 1-226)

Produktnummer ABIN3044520

Dieser Anti-Fatty Acid Synthase Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von Fatty Acid Synthase in WB, IHC, IF und ICC. Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für Fatty Acid Synthase Antikörper (AA 1-226) (ABIN3044520)

Target: Fatty Acid Synthase (FASN)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Fatty Acid Synthase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-Fatty Acid Synthase Antikörper

Bindungsspezifität: AA 1-226

Verwendungszweck: Anti-Fatty Acid Synthase/FASN Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Fatty Acid Synthase/FASN Antibody Picoband® (ABIN3044520). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human FASN recombinant protein (Position: M1-E226). Human FASN shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat FASN, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Immunofluorescence, 5 μg/mL
1. "Entrez Gene: FASN fatty acid synthase". 2. Jayakumar A, Tai MH, Huang WY, al-Feel W, Hsu M, Abu-Elheiga L, Chirala SS, Wakil SJ (Oct 1995). "Human fatty acid synthase: properties and molecular cloning". Proc Natl Acad Sci U S A 92 (19): 8695-9. 3. Smith S, Witkowski A, Joshi AK (July 2003). "Structural and functional organization of the animal fatty acid synthase". Prog. Lipid Res. 42 (4): 289-317.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Fatty Acid Synthase Antikörper (ABIN3044520)

Lu, Ma, Cai: "Increased HAGLR expression promotes non-small cell lung cancer proliferation and invasion via enhanced de novo lipogenesis." in: Tumour biology, Vol. 39, Issue 4, pp. 1010428317697570, (2017) (PubMed).

Details zu Fatty Acid Synthase

Target: Fatty Acid Synthase (FASN)

Andere Bezeichnung: FASN

Hintergrund:

Synonyms: Fatty acid synthase;2.3.1.85;[Acyl-carrier-protein] S-acetyltransferase;2.3.1.38;[Acyl-carrier-protein] S-malonyltransferase;2.3.1.39;3-oxoacyl-[acyl-carrier-protein] synthase;2.3.1.41;3-oxoacyl-[acyl-carrier-protein] reductase;1.1.1.100;3-hydroxyacyl-[acyl-carrier-protein] dehydratase;4.2.1.59;Enoyl-[acyl-carrier-protein] reductase;1.3.1.39;Oleoyl-[acyl-carrier-protein] hydrolase;3.1.2.14;FASN;FAS;

Tissue Specificity: Ubiquitous. Prominent expression in brain, lung, and liver. .

Background: Fatty acid synthase  (FAS) is an enzyme that in humans is encoded by the FASN gene. It is mapped to 17q25. The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Molekulargewicht: 273 kDa

Gen-ID: 2194

UniProt: P49327

Pathways: AMPK Signaling