CSTB Antikörper (Middle Region)

Produktnummer ABIN3044489

Produktdetails anti-CSTB Antikörper

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Bindungsspezifität: AA 26-45, Middle Region

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CSTB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Cystatin-B(CSTB) detection. Tested with WB, IHC-P in Human.

Sequenz: QLEEKENKKF PVFKAVSFKS

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Cystatin-B(CSTB) detection. Tested with WB, IHC-P in Human.
Gene Name: cystatin B(stefin B)
Protein Name: Cystatin-B

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Stefin B(26-45aa QLEEKENKKFPVFKAVSFKS).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CSTB is approximately 30 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu CSTB

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Andere Bezeichnung: CSTB (CSTB Produkte)

Synonyme: CST6 antikoerper, EPM1 antikoerper, EPM1A antikoerper, PME antikoerper, STFB antikoerper, ULD antikoerper, CSTB antikoerper, Cyb antikoerper, Epm1 antikoerper, Stfb antikoerper, MGC189005 antikoerper, pme antikoerper, cst6 antikoerper, epm1 antikoerper, stfb antikoerper, AA960480 antikoerper, ARABIDOPSIS THALIANA PHYTOCYSTATIN 6 antikoerper, ATCYS6 antikoerper, ATCYSB antikoerper, cystatin B antikoerper, LOC100008600 antikoerper, LOC100136235 antikoerper, CPI1 antikoerper, cystatin B antikoerper, cystatin B (stefin B) antikoerper, cystatin 14a, tandem duplicate 2 antikoerper, cystatin-B antikoerper, CSTB antikoerper, Cstb antikoerper, cstb antikoerper, cst14a.2 antikoerper, CYSB antikoerper, cpi1 antikoerper, LOC101123265 antikoerper

Hintergrund: Cystatin B(CSTB),also called STFB, is a small protein that is a member of the superfamily of cysteine protease inhibitors. It has been isolated from human spleen and liver and its amino acid sequence has been fully determined. The cystatin B gene is located on 21q22.3. It is widely distributed and is localized mostly intracellularly, but has been found extracellularly. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Its role is though to be as a protector against the proteinases leaking from lysosomes. A cystatin B multiprotein complex might have a specific cerebellar function, and that the loss of this function might contribute to the etiopathogenesis of EPM1. Upon differentiation to myotubes, CSTB becomes excluded from the nucleus and lysosomes, suggesting that the subcellular distribution of CSTB is dependent on the differentiation status of the cell.

Synonyms: CHROW21 antibody|CPI B antibody|CPI-B antibody|CST6 antibody|CSTB antibody|Cystatin B antibody|Cystatin-B antibody|CYTB antibody|CYTB_HUMAN antibody|EPM1 antibody|EPM1A antibody|Liver thiol proteinase inhibitor antibody|PME antibody|Stefin-B antibody|STFB antibody|ULD antibody

UniProt: P04080

Pathways: Response to Water Deprivation