TREM1 Antikörper (AA 74-90)

Produktnummer ABIN3044450

Produktdetails anti-TREM1 Antikörper

Target: TREM1 (Triggering Receptor Expressed On Myeloid Cells 1 (TREM1))

Bindungsspezifität: AA 74-90

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TREM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Triggering receptor expressed on myeloid cells 1(TREM1) detection. Tested with WB, IHC-P, IHC-F in Mouse.

Sequenz: FTRPSEVHMG KFTLKHD

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Triggering receptor expressed on myeloid cells 1(TREM1) detection. Tested with WB, IHC-P, IHC-F in Mouse.
Gene Name: triggering receptor expressed on myeloid cells 1
Protein Name: Triggering receptor expressed on myeloid cells 1(TREM-1)

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle of mouse TREM1(74-90aa FTRPSEVHMGKFTLKHD).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-TREM1 Antikörper (ABIN3044450)

Zhou, Xiong, Huang, Tang, Yu, Lan: "Identification of Genes Associated with Smad3-dependent Renal Injury by RNA-seq-based Transcriptome Analysis." in: Scientific reports, Vol. 5, pp. 17901, (2016) (PubMed).

Details zu TREM1

Target: TREM1 (Triggering Receptor Expressed On Myeloid Cells 1 (TREM1))

Andere Bezeichnung: TREM1 (TREM1 Produkte)

Hintergrund: Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide(LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8(IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1(MCP1, or SCYA2) and tumor necrosis factor(TNF), MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules(e.g., ITGB1) and costimulatory molecules(e.g., CD40). Trem1 is associated with DAP12(TYROBP), a molecule frequently associated with activating receptors.

Synonyms: CD354 antibody|OTTMUSP00000018206 antibody|TREM 1 antibody|TREM-1 antibody|TREM1 antibody|TREM1_HUMAN antibody|Triggering receptor expressed on monocytes 1 antibody|Triggering receptor expressed on myeloid cells 1 antibody|Triggering receptor TREM 1 antibody|Triggering receptor TREM1 antibody