CAMKK1 Antikörper (N-Term)

Produktnummer ABIN3044431

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch CAMKK1 in WB und IHC. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für CAMKK1 Antikörper (N-Term) (ABIN3044431)

Target: CAMKK1 (Calcium/calmodulin-Dependent Protein Kinase Kinase 1, alpha (CAMKK1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CAMKK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-CAMKK1 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Anti-CaMKK/CAMKK1 Antibody

Sequenz: QDPRAELVDR VAAINVAHLE EA

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Homologie: different from the related human sequence by three amino acids,and from the related rat sequence by two amino acids.

Produktmerkmale: Anti-CaMKK/CAMKK1 Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse CaMKK, different from the related human sequence by three amino acids, and from the related rat sequence by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Rat, MouseWestern blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu CAMKK1

Target: CAMKK1 (Calcium/calmodulin-Dependent Protein Kinase Kinase 1, alpha (CAMKK1))

Andere Bezeichnung: CAMKK1

Hintergrund:

Background: CAMKK1, Calcium/calmodulin-dependent protein kinase kinase 1 is an enzyme that in humans is encoded by the CAMKK1 gene. The CAMKK1 gene is mapped to chromosome 17. The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca (2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene.Camkk1binds calmodulin and activated Camk4 with a 6-fold increase in total activity and a 100-fold increase in Camk4 Ca (2+)-independent activity. Camkk1 catalyzed a 10-fold increase in the total activity of Camk1 and had no effect on Camk2. Cotransfection of COS-7 cells with Camkk1 and Camk4 resulted in a 14-fold increase in CRE-binding protein-dependent transcription compared with Camk4 alone, suggesting that Camkk1 enhances Camk4-mediated transcriptional regulation.

Gene Full Name: calcium/calmodulin-dependent protein kinase kinase 1, alpha

Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Molekulargewicht: 56 kDa

Gen-ID: 55984

UniProt: Q8VBY2

Pathways: AMPK Signaling