NOS1 Antikörper (C-Term)

Produktnummer ABIN3044371

Produktdetails anti-NOS1 Antikörper

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Bindungsspezifität: AA 1418-1434, C-Term

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Verwendungszweck: Anti-nNOS (neuronal)/NOS1 Antibody Picoband®

Sequenz: IAFIEESKKD TDEVFSS

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-nNOS (neuronal)/NOS1 Antibody (ABIN3044371). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human nNOS(neuronal), identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Rat, Human, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. "Entrez Gene: NOS1 Nitric oxide synthase 1 (neuronal)". 2. Shteyer, E., Edvardson, S., Wynia-Smith, S. L., Pierri, C. L., Zangen, T., Hashavya, S., Begin, M., Yaacov, B., Cinamon, Y., Koplewitz, B. Z., Vromen, A., Elpeleg, O., Smith, B. C. Truncating mutation in the nitric oxide synthase 1 gene is associated with infantile achalasia. Gastroenterology 148: 533-536, 2015. 3. Xu, W., Gorman, P., Sheer, D., Bates, G., Kishimoto, J., Lizhi, L., Emson, P. Regional localization of the gene coding for human brain nitric oxide synthase (NOS1) to 12q24.2-24.31 by fluorescent in situ hybridization. Cytogenet. Cell Genet. 64: 62-63, 1993.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-NOS1 Antikörper (ABIN3044371)

Yin, Liang, Wang, Yan, Yin, Wu, Su: "Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation." in: International journal of molecular medicine, Vol. 41, Issue 2, pp. 649-658, (2018) (PubMed).

Ding, Tang, Tang, Zhang, Wang: "A Variant in the Precursor of MicroRNA-146a is Responsible for Development of Erectile Dysfunction in Patients with Chronic Prostatitis via Targeting NOS1." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 929-937, (2017) (PubMed).

Zheng, Sulieman, Li, Wei, Xu, Shi: "Nitric oxide and thyroid hormone receptor alpha 1 contribute to ovarian follicular development in immature hyper- and hypo-thyroid rats." in: Reproductive biology, Vol. 15, Issue 1, pp. 27-33, (2015) (PubMed).

Kong, Wei, Fedail, Shi, Nagaoka, Watanabe: "Effects of thyroid hormones on the antioxidative status in the uterus of young adult rats." in: The Journal of reproduction and development, Vol. 61, Issue 3, pp. 219-27, (2015) (PubMed).

Li, Zhou, Wei, Huang, Shi: "Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and soluble guanylyl cyclase ? and ? subunits in postnatal porcine uteri." in: Acta histochemica, Vol. 116, Issue 3, pp. 466-73, (2014) (PubMed).

Xu, Wei, Zheng, Mao, Zheng, Li, Shi: "Protective effects of Big-leaf mulberry and physiological roles of nitric oxide synthases in the testis of mice following water immersion and restraint stress." in: Acta histochemica, Vol. 116, Issue 8, pp. 1323-30, (2014) (PubMed).

Liu, Li, Zhang, Fu, Liu, Zhou: "Activity and expression of nitric oxide synthase in pork skeletal muscles." in: Meat science, Vol. 99, pp. 25-31, (2014) (PubMed).

Fedail, Zheng, Wei, Kong, Shi: "Roles of thyroid hormones in follicular development in the ovary of neonatal and immature rats." in: Endocrine, Vol. 46, Issue 3, pp. 594-604, (2014) (PubMed).

Ding, Zhang, Hui, Zhang, Zhang, Li, Shi: "Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and soluble guanylyl cyclase ?1 and ?1 subunits in the ovary of fetal, neonatal and immature pigs." in: Animal reproduction science, Vol. 131, Issue 3-4, pp. 172-80, (2012) (PubMed).

Chang, Xiao, Wang, Yan, Yang, Wang: "2, 3, 5, 4'-Tetrahydroxystilbene-2-O-beta-D-glucoside improves gastrointestinal motility disorders in STZ-induced diabetic mice." in: PLoS ONE, Vol. 7, Issue 12, pp. e50291, (2012) (PubMed).

Zhang, Wei, Wang, Ding, Wang, Shi: "Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and the related nitric oxide/cyclic GMP signaling pathway in the ovaries of neonatal and immature rats." in: Journal of Zhejiang University. Science. B, Vol. 12, Issue 1, pp. 55-64, (2011) (PubMed).

Wang, Yan, Zhao, Wu, Zhou: "The diversity of nNOS gene expression in avulsion-injured spinal motoneurons among laboratory rodents." in: Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, Vol. 22, Issue 1, pp. 37-42, (2010) (PubMed).

Wu, Yin, Yao, Haefliger: "Inhibition by brimonidine of forskolin-induced nitric oxide synthase expression in human ciliary bodies in vitro." in: Molecular vision, Vol. 13, pp. 493-6, (2007) (PubMed).

Yuan, Liu, Zhang, Yuan, Muteliefu, Lu: "Upregulated expression of neuronal nitric oxide synthase by insulin in both neurons and astrocytes." in: Brain research, Vol. 1008, Issue 1, pp. 1-10, (2004) (PubMed).

Chen, Qian, Zhang, Chang, Duan: "Distribution of constitutive nitric oxide synthase in the jejunum of adult rat." in: World journal of gastroenterology, Vol. 8, Issue 3, pp. 537-9, (2002) (PubMed).

Details zu NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Andere Bezeichnung: NOS1 (NOS1 Produkte)

Hintergrund:

Synonyms: Nitric oxide synthase, brain,1.14.13.39,Constitutive NOS,NC-NOS,N

Tissue Specificity: Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.

Background: Nitric oxide synthase 1 (neuronal), also known as NOS1, is an enzyme that in humans is encoded by the NOS1 gene. The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.

Sequence Similarities: Belongs to the NOS family.

Molekulargewicht: 160 kDa

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction