Apoptosis Inhibitor 5 Antikörper (C-Term)

Produktnummer ABIN3044316

Produktdetails anti-Apoptosis Inhibitor 5 Antikörper

Target: Apoptosis Inhibitor 5 (API5)

Bindungsspezifität: AA 487-504, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Apoptosis Inhibitor 5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Verwendungszweck: Anti-Apoptosis inhibitor 5/API5 Antibody Picoband®

Sequenz: KYSSNLGNFN YERSLQGK

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Apoptosis inhibitor 5/API5 Antibody (ABIN3044316). Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Apoptosis inhibitor 5, different from the related mouse sequence by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat, Human, Mouse
Immunocytochemistry , 0.5-1 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. Hartz, P. A.Personal Communication.Baltimore, Md. 12/12/2005. 2. Tewari, M., Yu, M., Ross, B., Dean, C., Giordano, A., Rubin, R. : AAC-11, a novel cDNA that inhibits apoptosis after growth factor withdrawal. Cancer Res. 57: 4063-4069, 1997.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Apoptosis Inhibitor 5

Target: Apoptosis Inhibitor 5 (API5)

Andere Bezeichnung: API5 (API5 Produkte)

Hintergrund:

Synonyms: Apoptosis inhibitor 5,API-5,Antiapoptosis clone 11 protein,AAC-11,Cell migration-inducing gene 8 protein,Fibroblast growth factor 2-interacting factor,FIF,Protein XAGL,API5,MIG8,

Tissue Specificity: Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer. .

Background: Many growth factors and cytokines act as cellular survival factors by preventing programmed cell death (apoptosis). Apoptosis inhibitor 5 (API5) is an antiapoptotic factor which may have a role in protein assembly. The API5 gene to chromosome 11p12 based on an alignment of the API5 sequence with the genomic sequence It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription.

Sequence Similarities: Belongs to the API5 family.

Molekulargewicht: 59 kDa

Pathways: Growth Factor Binding