Hsc70 Antikörper (N-Term)

Produktnummer ABIN3044300

Es sind 5+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-Hsc70 Antikörper wird verwendet zum Nachweis von Hsc70 in Proben von Human, Ratte und Maus. Er wurde validiert für WB, IHC, ICC, IF, FACS und IHC (fro).

Kurzübersicht für Hsc70 Antikörper (N-Term) (ABIN3044300)

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Hsc70 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Hsc70 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Anti-Hsp70/HSPA1A/HSPA1B Antibody

Sequenz: TTYSCVGVFQ HGKVEIIAN

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Homologie: identical to the related rat,mouse sequence.

Produktmerkmale: Anti-Hsp70/HSPA1A/HSPA1B Antibody. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Hsp70, identical to the related rat and mouse sequence.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse, Rat Immunocytochemistry , 0.5-1 μg/mL, Human Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-Hsc70 Antikörper (ABIN3044300)

Zhang, Tang, Li, Li, Zhang, Yan, Cui: "Effects of intracerebral hemorrhage and subsequent minimally invasive hematoma aspiration on expression of apoptosisrelated genes in rats." in: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 5371-8, (2016) (PubMed).

Pu, Shi, Wang, Wang, Guo, Liu, Sun, Gao, Zhou: "Effects of minocycline on the expression of NGF and HSP70 and its neuroprotection role following intracerebral hemorrhage in rats." in: Journal of biomedical research, Vol. 25, Issue 4, pp. 292-8, (2013) (PubMed).

Li, Roubeix, Wang, Shi, Liu, Baudouin, Chen: "Therapeutic efficacy of trehalose eye drops for treatment of murine dry eye induced by an intelligently controlled environmental system." in: Molecular vision, Vol. 18, pp. 317-29, (2012) (PubMed).

Yi, Peng, Chang, Peng, Yan, Lin: "Effect of pre-moxibustion on apoptosis and proliferation of gastric mucosa cells." in: World journal of gastroenterology, Vol. 13, Issue 15, pp. 2174-8, (2007) (PubMed).

Wu, Cao, Gao, Chen, Wang, Zumbika, Qian: "Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells." in: World journal of gastroenterology, Vol. 10, Issue 20, pp. 2944-8, (2004) (PubMed).

Details zu Hsc70

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Andere Bezeichnung: HSPA8

Hintergrund:

Background: The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. All of the Hsp70 proteins have three major functional domains: An N-terminal ATPase domain binds ATP (Adenosine triphosphate) and hydrolyzes it to ADP (Adenosine diphosphate); A substrate binding domain contains a groove with an affinity for neutral, hydrophobic amino acid residues; A C-terminal domain rich in alpha helical structure acts as a 'lid' for the substrate binding domain. By binding tightly to partially-synthesized peptide sequences (incomplete proteins), Hsp70 prevents them from aggregating and being rendered nonfunctional. And it also can act to protect cells from thermal or oxidative stress. Finally, Hsp70 seems to be able to participate in disposal of damaged or defective proteins. Interaction with CHIP (Carboxyl-terminus of Hsp70 Interacting Protein)–an E3 ubiquitin ligase–allows Hsp70 to pass proteins to the cell's ubiquitination and proteolysis pathways.

Gene Full Name: heat shock protein family A (Hsp70) member 1A/1B

Sequence Similarities: Belongs to the beta-catenin family.

Molekulargewicht: 70 kDa

Gen-ID: 3303, 3304

UniProt: P0DMV8