PEBP1 Antikörper (N-Term)

Produktnummer ABIN3044276

Produktdetails anti-PEBP1 Antikörper

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Bindungsspezifität: AA 15-29, N-Term

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PEBP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Phosphatidylethanolamine-binding protein 1(PEBP1) detection. Tested with WB, IHC-P in Mouse,Rat.

Sequenz: QEVDEPPQHA LRVDY

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Phosphatidylethanolamine-binding protein 1(PEBP1) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: phosphatidylethanolamine binding protein 1
Protein Name: Phosphatidylethanolamine-binding protein 1(PEBP-1)

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse PBP(15-29aa QEVDEPPQHALRVDY), identical to the related rat sequence.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu PEBP1

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Andere Bezeichnung: PEBP1 (PEBP1 Produkte)

Synonyme: HCNP antikoerper, HCNPpp antikoerper, PBP antikoerper, PEBP antikoerper, PEBP-1 antikoerper, RKIP antikoerper, pbp antikoerper, hcnp antikoerper, pebp antikoerper, rkip antikoerper, Pbp antikoerper, Pbp1 antikoerper, Pbpr antikoerper, Rkip antikoerper, PEBP1 antikoerper, BcDNA:LP12095 antikoerper, CG18594 antikoerper, Dmel\\CG18594 antikoerper, T2 antikoerper, zgc:56033 antikoerper, zgc:76942 antikoerper, phosphatidylethanolamine binding protein 1 antikoerper, phosphatidylethanolamine binding protein 1 L homeolog antikoerper, phosphatidylethanolamine-binding protein antikoerper, phosphatidylethanolamine-binding protein,putative antikoerper, Phosphatidylethanolamine-binding protein 1 antikoerper, PEBP1 antikoerper, pebp1 antikoerper, Pebp1 antikoerper, pebp1.L antikoerper, RR_RS07785 antikoerper, PVX_235290 antikoerper, PVX_123630 antikoerper, PKH_143640 antikoerper, EAM_1207 antikoerper

Hintergrund: PEBP1(Phosphatidylethanolamine-binding protein 1), also called PBP, RKIP, inhibits the phosphorylation and activation of MEK by RAF1. PEBP1 is identical to the phosphatidylethanolamine-binding protein(PBP) with a relative molecular mass of 23 kD. The PEBP1 gene is mapped on 12q24.23. PEBP1 coimmunoprecipitates with RAF1 and MEK from cell lysates and colocalizes with RAF1 when examined by confocal microscopy. PEBP1 overexpression interferes with the activation of MEK and ERK, induction of AP1-dependent reporter genes, and transformation elicited by an oncogenically activated RAF1 kinase. PEBP1 expression was rapidly upregulated during induction of chemotherapy-triggered apoptosis in human prostate and breast cancer cell lines, and maximal RKIP expression correlated perfectly with the onset of apoptosis by Chatterjee et al(2004). RKIP depletion decreased the mitotic index, the number of metaphase cells, traversal times from nuclear envelope breakdown to anaphase, and an override of mitotic checkpoints induced by spindle poisons.

Synonyms: HCNP antibody|HCNPpp antibody|Hippocampal cholinergic neurostimulating peptide antibody|Neuropolypeptide h3 antibody|PEBP antibody|PEBP 1 antibody|PEBP-1 antibody|PEBP1 antibody|PEBP1_HUMAN antibody|Phosphatidylethanolamine binding protein antibody|Phosphatidylethanolamine binding protein 1 antibody|Prostatic binding protein antibody|Prostatic-binding protein antibody|R kip antibody|Raf kinase inhibitor protein antibody|Raf kinase inhibitory protein antibody|RKIP antibody

UniProt: P70296

Pathways: Feeding Behaviour