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Netrin 1 Antikörper (N-Term)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Netrin 1 in WB. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 2+ Publikationen erwähnt.
Produktnummer ABIN3044248

Kurzübersicht für Netrin 1 Antikörper (N-Term) (ABIN3044248)

Target

Alle Netrin 1 (NTN1) Antikörper anzeigen
Netrin 1 (NTN1)

Reaktivität

  • 50
  • 42
  • 34
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 62
  • 8
  • 4
  • 2
  • 1
  • 1
  • 1
Kaninchen

Klonalität

  • 68
  • 11
Polyklonal

Konjugat

  • 47
  • 7
  • 6
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Netrin 1 Antikörper ist unkonjugiert

Applikation

  • 61
  • 33
  • 19
  • 18
  • 15
  • 13
  • 13
  • 6
  • 5
  • 4
  • 3
  • 2
  • 1
Western Blotting (WB)
  • Bindungsspezifität

    • 15
    • 7
    • 6
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 32-51, N-Term

    Verwendungszweck

    Anti-Netrin 1/NTN1 Antibody Picoband®

    Sequenz

    AGQAAQPDPC SDENGHPRRC

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-Netrin 1/NTN1 Antibody (ABIN3044248). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the N-terminus of human Netrin 1, identical to the related rat and mouse sequences.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
    1. Colon-Ramos, D. A., Margeta, M. A., Shen, K. Glia promote local synaptogenesis through UNC-6 (netrin) signaling in C. elegans. Science 318: 103-106, 2007. 2. Corset, V., Nguyen-Ba-Charvet, K. T., Forcet, C., Moyse, E., Chedotal, A., Mehlen, P. Netrin-1-mediated axon outgrowth and cAMP production requires interaction with adenosine A2b receptor. Nature 407: 747-750, 2000. 3. Powell AW, Sassa T, Wu Y, Tessier-Lavigne M, Polleux F (May 2008). Ghosh, Anirvan. ed. "Topography of thalamic projections requires attractive and repulsive functions of Netrin-1 in the ventral telencephalon."

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

    Konservierungsmittel

    Thimerosal (Merthiolate), Sodium azide

    Vorsichtsmaßnahmen

    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Qian-hua, Shao-ping, Jian-wen, Yun, Li: "Reduced expression of netrin-1 is associated with fetal growth restriction." in: Molecular and cellular biochemistry, Vol. 350, Issue 1-2, pp. 81-7, (2011) (PubMed).

    Zhong, Zou, Zhao, Hu, Xie: "Effect of different concentrations of neogenin on proliferation, apoptosis and related proliferative factors in human trophoblasts." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 30, Issue 4, pp. 500-4, (2010) (PubMed).

  • Target

    Netrin 1 (NTN1)

    Andere Bezeichnung

    NTN1

    Hintergrund

    Synonyms: Netrin-1,Epididymis tissue protein Li 131P,NTN1,NTN1L,

    Tissue Specificity: Widely expressed in normal adult tissues with highest levels in heart, small intestine, colon, liver and prostate. Reduced expression in brain tumors and neuroblastomas. Expressed in epididymis (at protein level). .

    Background: Netrin-1 (NTN1), also known as NTN1L, is a diffusible protein made by floor plate cells. Netrin-1 is found in the floor plate and neuroepithelial cells of the ventral region of the spinal cord, as well as other locations in the nervous system including the somatic mesoderm, pancreas and cardiac muscle. The human NTN1 gene is mapped to chromosome 17p13-p12 by FISH. Netrin-1 can attract spinal commissural axons and repel trochlear axons in vitro. And netrin-1 expression confers a selective advantage for tumor cell survival. The binding of netrin-1 to DCC appears to depend on the presence of a coreceptor or accessory proteins. Adenosine A2b receptor is actually a netrin-1 receptor and induces cAMP accumulation on binding netrin-1, and that netrin-1-dependent outgrowth of dorsal spinal cord axons ly involves A2b. Netrin-1 can promote intestinal tumor development, probably by regulating cell survival. Thus, a netrin-1 receptor or receptors function as conditional tumor suppressors. Expression of Unc5b triggered endothelial cell repulsion in response to netrin-1 in vitro, whereas a truncated Unc5b lacking the intracellular signaling domain failed to induce repulsion.

    Sequence Similarities: Contains 3 laminin EGF-like domains.

    Molekulargewicht

    8 kDa

    UniProt

    O95631

    Pathways

    Regulation of Cell Size
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