Peroxiredoxin 3 Antikörper (C-Term)

Produktnummer ABIN3044200

Produktdetails anti-Peroxiredoxin 3 Antikörper

Target: Peroxiredoxin 3 (PRDX3)

Bindungsspezifität: AA 239-256, C-Term

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Peroxiredoxin 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Thioredoxin-dependent peroxide reductase, mitochondrial(PRDX3) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Sequenz: TIKPSPAASK EYFQKVNQ

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Thioredoxin-dependent peroxide reductase, mitochondrial(PRDX3) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: peroxiredoxin 3
Protein Name: Thioredoxin-dependent peroxide reductase, mitochondrial

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Peroxiredoxin 3(239-256aa TIKPSPAASKEYFQKVNQ), different from the related mouse and rat sequences by three amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu Peroxiredoxin 3

Target: Peroxiredoxin 3 (PRDX3)

Andere Bezeichnung: PRDX3 (PRDX3 Produkte)

Synonyme: cb718 antikoerper, wu:fk49e09 antikoerper, zgc:110282 antikoerper, zgc:112512 antikoerper, MGC83969 antikoerper, PRDX3 antikoerper, AOP-1 antikoerper, AOP1 antikoerper, HBC189 antikoerper, MER5 antikoerper, PRO1748 antikoerper, SP-22 antikoerper, prx-III antikoerper, AW822249 antikoerper, Aop1 antikoerper, D0Tohi1 antikoerper, Ef2l antikoerper, Mer5 antikoerper, Prx3 antikoerper, SP22 antikoerper, TDXM antikoerper, MTAP antikoerper, peroxiredoxin 3 antikoerper, peroxiredoxin 3 S homeolog antikoerper, peroxiredoxin PRX3 antikoerper, prdx3 antikoerper, PRDX3 antikoerper, prdx3.S antikoerper, PRX3 antikoerper, Prdx3 antikoerper

Hintergrund: PRDX3(peroxiredoxin 3) also known as AOP-1, MER5, SP-22 or PRX3, is localized exclusively in mitochondria. The deduced 256-amino acid human AOP1 protein shares 86 % amino acid sequence similarity with mouse Aop1, and significant similarity with both the human proliferation-associated gene A product and the mouse stress-induced peritoneal macrophage protein Msp23. The PRDX3 gene is mapped on 10q26.11. Expression of PRDX3 is induced by MYC and is reduced in c-myc -/- cells. Chromatin immunoprecipitation analysis spanning the entire PRDX3 genomic sequence revealed that MYC binds preferentially to a 930-bp region surrounding exon 1. Results using mitochondria-specific fluorescent probes demonstrated that PRDX3 is essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells. These data provided evidence that PRDX3 is a MYC target gene that is required to maintain normal mitochondrial function.

Synonyms: Antioxidant Protein 1 antibody|AOP 1 antibody|AOP-1 antibody|AOP1 antibody|HBC189 antibody|MER 5 antibody|MER5 antibody|MGC104387 antibody|MGC24293 antibody|mitochondrial antibody|Mitochondrial thioredoxin dependent peroxide reductase antibody|peroxiredoxin 3 antibody|PeroxiredoxinIII antibody|Peroxiredoxin-3 antibody|Peroxiredoxin3 antibody|PRDX 3 antibody|PRDX3 antibody|PRDX3_HUMAN antibody|PRO1748 antibody|Protein MER5 homolog antibody|PRX III antibody|PRXIII antibody|Prx-III antibody|PRX3 antibody|SP 22 antibody|SP-22 antibody|SP22 antibody|Thioredoxin dependent peroxide reductase mitochondrial antibody|Thioredoxin dependent peroxide reductase precursor antibody|Thioredoxin-dependent peroxide reductase antibody

UniProt: P30048

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process