Peroxiredoxin 3 Antikörper (C-Term)

Produktnummer ABIN3044200

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Peroxiredoxin 3 in WB, IHC, IF, ICC und FACS. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus.

Kurzübersicht für Peroxiredoxin 3 Antikörper (C-Term) (ABIN3044200)

Target: Peroxiredoxin 3 (PRDX3)

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Peroxiredoxin 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-Peroxiredoxin 3 Antikörper

Bindungsspezifität: AA 239-256, C-Term

Verwendungszweck: Anti-Peroxiredoxin 3/PRDX3 Antibody Picoband®

Sequenz: TIKPSPAASK EYFQKVNQ

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Peroxiredoxin 3/PRDX3 Antibody (ABIN3044200). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Peroxiredoxin 3, different from the related mouse and rat sequences by three amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
1. Chiribau, C. B., Cheng, L., Cucoranu, I. C., Yu, Y.-S., Clempus, R. E., Sorescu, D. FOXO3A regulates peroxiredoxin III expression in human cardiac fibroblasts. J. Biol. Chem. 283: 8211-8217, 2008. 2. Nemoto, Y., Yamamoto, T., Takada, S., Matsui, Y., Obinata, M. Antisense RNA of the latent period gene (MER5) inhibits the differentiation of murine erythroleukemia cells. Gene 91: 261-265, 1990. 3. Wonsey, D. R., Zeller, K. I., Dang, C. V. The c-Myc target gene PRDX3 is required for mitochondrial homeostasis and neoplastic transformation. Proc. Nat. Acad. Sci. 99: 6649-6654, 2002.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Peroxiredoxin 3

Target: Peroxiredoxin 3 (PRDX3)

Andere Bezeichnung: PRDX3

Hintergrund:

Synonyms: Thioredoxin-dependent peroxide reductase, mitochondrial;1.11.1.15;Antioxidant protein 1;AOP-1;HBC189;Peroxiredoxin III;Prx-III;Peroxiredoxin-3;Protein MER5 homolog;PRDX3;AOP1;

Background: PRDX3 (peroxiredoxin 3) also known as AOP-1, MER5, SP-22 or PRX3, is localized exclusively in mitochondria. The deduced 256-amino acid human AOP1 protein shares 86% amino acid sequence similarity with mouse Aop1, and significant similarity with both the human proliferation-associated gene A product and the mouse stress-induced peritoneal macrophage protein Msp23. The PRDX3 gene is mapped on 10q26.11. Expression of PRDX3 is induced by MYC and is reduced in c-myc -/- cells. Chromatin immunoprecipitation analysis spanning the entire PRDX3 genomic sequence revealed that MYC binds preferentially to a 930-bp region surrounding exon 1. Results using mitochondria-specific fluorescent probes demonstrated that PRDX3 is essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells. These data provided evidence that PRDX3 is a MYC target gene that is required to maintain normal mitochondrial function.

Sequence Similarities: Belongs to the AhpC/TSA family.

Molekulargewicht: 26 kDa

UniProt: P30048

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process