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NME2 Antikörper (C-Term)

NME2 Reaktivität: Human, Maus, Ratte WB, IHC, IF, ICC, FACS Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3044198
  • Target Alle NME2 Antikörper anzeigen
    NME2 (Nucleoside diphosphate kinase B (NME2))
    Bindungsspezifität
    • 15
    • 8
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 137-152, C-Term
    Reaktivität
    • 53
    • 37
    • 8
    • 2
    • 1
    • 1
    • 1
    Human, Maus, Ratte
    Wirt
    • 44
    • 9
    • 1
    Kaninchen
    Klonalität
    • 43
    • 11
    Polyklonal
    Konjugat
    • 29
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser NME2 Antikörper ist unkonjugiert
    Applikation
    • 31
    • 19
    • 17
    • 13
    • 13
    • 8
    • 6
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    Verwendungszweck
    Anti-NME2 Antibody Picoband®
    Sequenz
    EELVDYKSCA HDWVYE
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins
    Produktmerkmale
    Anti-NME2 Antibody (ABIN3044198). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence at the C-terminus of human NME2, different from the related mouse and rat sequences by one amino acid.
    Isotyp
    IgG
    Top Product
    Discover our top product NME2 Primärantikörper
  • Applikationshinweise
    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry , 0.5-1 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Backer, J. M., Mendola, C. E., Kovesdi, I., Fairhurst, J. L., O'Hara, B., Eddy, R. L., Jr., Shows, T. B., Mathew, S., Murty, V. V. V. S., Chaganti, R. S. K. Chromosomal localization and nucleoside diphosphate kinase activity of human metastasis-suppressor genes NM23-1 and NM23-2.Oncogene 8: 497-502, 1993. 2. Masse, K., Dabernat, S., Bourbon, P.-M., Larou, M., Amrein, L., Barraud, P., Perel, Y., Camara, M., Landry, M., Lacombe, M.-L., Daniel, J.-Y.Characterization of the nm23-M2, nm23-M3 and nm23-M4 mouse genes: comparison with their human homologs. Gene 296: 87-97, 2002. 3. Srivastava, S., Li, Z., Ko, K., Choudhury, P., Albaqumi, M., Johnson, A. K., Yan, Y., Backer, J. M., Unutmaz, D., Coetzee, W. A., Skolnik, E. Y.Histidine phosphorylation of the potassium channel KCa3.1 by nucleoside diphosphate kinase B is required for activation of KCa3.1 and CD4 T cells. Molec. Cell 24: 665-675, 2006.
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #300030 (Immunohistochemistry)
    'Independent Validation' Siegel
    by
    Human Protein Atlas
    No.
    #300030
    Datum
    13.12.2016
    Antigen
    P18
    Chargennummer
    Validierte Anwendung
    Immunohistochemistry
    Positivkontrolle
    tonsil, urinary bladder
    Negativkontrolle
    Bewertung
    Passed. ABIN3044198 staining is consistent with staining pattern of other commercial P18/NME2 antibodies.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN3044198
    Sekundärantikörper
    HRP polymer (ThermoFisher Scientific, TL-125-PH)
    Full Protocol
    • Sample preparation:
      • Fixation and TMA preparation according to PMID: 22688270.
      • Cut 4µm TMA sections using a waterfall microtome (ThermoFisher Scientific, HM355S).
      • Dry sections ON at RT and then back them for 12-24h at 50°C.
    • Deparaffinization and rehydration in xylene and graded alcohol series:
      • xylene twice for 3min.
      • 100% ethanol for 1min.
      • 95% ethanol for 1min.
      • 0.3% H2O2 in 95% ethanol for 5min to block endogenous peroxidase.
      • 70% ethanol for 1min.
      • 50% ethanol for 1min.
      • distilled H2O for 2min.
    • Antigen Retrieval by Heat Induced Epitope Retrieval (HIER):
      • Boil the sections in Lab Vision Citrate buffer pH6.0 (ThermoFisher Scientific, TA-250-PM1X) for 4min at 125°C in a decloaking chamber (Biocare Medical).
      • Allow slides to cool down to 90°C in the decloaking chamber.
    • Immunostaining in a Lab Vision Autostainer 480 (ThermorFisher Scientific) at RT with 300µl of reagents for each step:
      • Rinse sections in Lab Vision wash buffer with extra tween added to a final concentration of 0.2% (ThermoFisher Scientific, TA-999-TT and TA-125-TW) wash buffer.
      • Block sections with Lab Vision Ultra V-Block (ThermoFisher Scientific, TA-125-UB) for 5min.
      • Rinse sections 2x in wash buffer.
      • Incubate sections with primary rabbit anti-P18/NME2 antibody (antibodies-online, ABIN3044198) diluted 1:25000 for 30min.
      • Rinse sections 3x in wash buffer.
      • Incubate sections with labeled HRP polymer (ThermoFisher Scientific, TL-125-PH) for 30min.
      • Rinse sections 2x in wash buffer.
      • Develop in DAB Quanto solution (ThermoFisher Scientific, TL-125-QHDX) for 5min.
      • Rinse sections in distilled H2O.
    • Washing, counterstaining, and coverslipping in Autostainer XL (Leica Biosystems, ST5010) at RT:
      • Counterstaining in Mayer’s hematoxylin plus (HistoLab, 01820) for 7.5min.
      • Rinse sections in tap water for 5min.
      • Rinse sections in lithium carbonate water diluted 1:5 for 5min.
      • Dehydration in graded ethanol series and Neo-Clear (Merck Millipore, 109843) according to the manufacturer recommendations.
      • Automated mounting (Leica Biosystems, CV5030) of coverslip with Pertex (Histolab, 00871).
    • Microscopy:
      • Image acquisition on a Leica Aperio AT2 and Aperio Scanscope AT at 20x magnification.
    Anmerkungen
    ABIN3044198 shows staining in most tissues similar to alternative P18/NME2 antibodies. Determination of the appropriate dilution factor proved difficult.
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    Konservierungsmittel
    Thimerosal (Merthiolate), Sodium azide
    Vorsichtsmaßnahmen
    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    NME2 (Nucleoside diphosphate kinase B (NME2))
    Andere Bezeichnung
    NME2 (NME2 Produkte)
    Hintergrund

    Synonyms: Nucleoside diphosphate kinase B;NDK B;NDP kinase B;2.7.4.6;C-myc purine-binding transcription factor PUF;Histidine protein kinase NDKB;2.7.13.3;nm23-H2;NME2;NM23B;

    Tissue Specificity: Ubiquitously expressed. .

    Background: NME2 (NME/NM23 nucleoside diphosphate kinase 2) also called non-metastatic cells 2, protein (NM23B) expressed in, NM23-H2, NM23B or NDPKB, is identical to the beta subunit of human erythrocyte NDP kinase. Little to no expression was detected in other mouse tissues examined. Using Northern blot analysis, Masse et al. (2002) detected high expression of mouse Nme2, which they called nm23-M2, in heart, liver, and kidney, with intermediate expression in skeletal muscle. In situ hybridization of 15-day postcoitum mouse embryos showed ubiquitous Nme2 expression. The mouse and human NME2 genes contain 5 exons and span about 6.0 kb. The NME2 gene is mapped on 17q21.33. Srivastava et al. (2006) concluded that histidine phosphorylation regulates KCa3.1 channel activity and that NDPBK is critical to the channel activity and the activation of CD4 T cells.

    Sequence Similarities: Belongs to the NDK family.

    Molekulargewicht
    17 kDa
    UniProt
    P22392
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