HSPB2 Antikörper (Middle Region)

Produktnummer ABIN3044185

Produktdetails anti-HSPB2 Antikörper

Target: HSPB2 (Heat Shock 27kDa Protein 2 (HSPB2))

Bindungsspezifität: AA 102-118, Middle Region

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSPB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Verwendungszweck: Anti-Heat shock protein beta-2 HSPB2 Antibody Picoband®

Sequenz: RHPQRLDRHG FVSREFC

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Heat shock protein beta-2 HSPB2 Antibody (ABIN3044185). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human HSPB2, identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat, Human, Mouse
Immunocytochemistry , 0.5-1 μg/mL, Human, Mouse, Rat
1. Iwaki, A., Nagano, T., Nakagawa, M., Iwaki, T., Fukumaki, Y. Identification and characterization of the gene encoding a new member of the alpha-crystallin/Small hsp family, closely linked to the alpha-B-crystallin gene in a head-to-head manner. Genomics 45: 386-394, 1997.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(F) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu HSPB2

Target: HSPB2 (Heat Shock 27kDa Protein 2 (HSPB2))

Andere Bezeichnung: HSPB2 (HSPB2 Produkte)

Hintergrund:

Synonyms: Heat shock protein beta-2,HspB2,DMPK-binding protein,MKBP,HSPB2,

Tissue Specificity: Expressed preferentially in skeletal muscle and heart but not in the lens.

Background: Alpha-B-crystallin is a member of the alpha-crystallin/small heat-shock protein (HSP) family and under various neuropathologic conditions accumulates in reactive astrocytes and degenerating neurons. In the 5-prime flanking region of the CRYAB gene on 11q22-q23, where a constitutive DNaseI hypersensitive site is located, Iwaki et al. (1997) identified a gene transcribed in the opposite ion. Analysis of its mRNA structure by RT-PCR and 5-prime/3-prime RACE revealed that this gene is composed of 2 exons and encodes a new member of the alpha-crystallin/hsp family. They termed the gene HSPB2. Iwaki et al. (1997) also determined the complete genomic structure of the rat homolog of HSPB2. Southern blot analysis demonstrated that HSPB2is expressed preferentially in skeletal muscle and heart but not in the lens, while the neighboring CRYAB gene is highly expressed in all 3 tissues. The 2 related genes are arranged in a head-to-head manner with an intergenic sequence of less than 1 kb, raising a possibility of shared regulatory elements for their expression.

Sequence Similarities: Belongs to the small heat shock protein (HSP20) family.

Molekulargewicht: 23 kDa

UniProt: Q16082