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Hexosaminidase A Antikörper (Middle Region)

HEXA Reaktivität: Human, Maus, Ratte WB, IHC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3044173
  • Target Alle Hexosaminidase A (HEXA) Antikörper anzeigen
    Hexosaminidase A (HEXA)
    Bindungsspezifität
    • 8
    • 8
    • 8
    • 7
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 191-207, Middle Region
    Reaktivität
    • 55
    • 22
    • 14
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte
    Wirt
    • 45
    • 13
    Kaninchen
    Klonalität
    • 47
    • 11
    Polyklonal
    Konjugat
    • 37
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Hexosaminidase A Antikörper ist unkonjugiert
    Applikation
    • 46
    • 32
    • 10
    • 7
    • 7
    • 3
    • 3
    • 3
    • 3
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC)
    Verwendungszweck
    Anti-HEXA Antibody Picoband®
    Sequenz
    DVMAYNKLNV FHWHLVD
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins
    Produktmerkmale
    Anti-HEXA Antibody (ABIN3044173). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence in the middle region of human HEXA, different from the related rat and mouse sequences by one amino acid.
    Isotyp
    IgG
    Top Product
    Discover our top product HEXA Primärantikörper
  • Applikationshinweise
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
    Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
    1. Akli, S., Chomel, J.-C., Lacorte, J.-M., Bachner, L., Poenaru, A., Poenaru, L. Ten novel mutations in the HEXA gene in non-Jewish Tay-Sachs patients. Hum. Molec. Genet. 2: 61-67, 1993. 2. Beutler, E., Kuhl, W., Comings, D. Hexosaminidase isozyme in type O Gm2 gangliosidosis (Sandhoff-Jatzkewitz disease). Am. J. Hum. Genet. 27: 628-638, 1975. 3. Chern, C. J., Beutler, E., Kuhl, W., Gilbert, F., Mellman, W. J., Croce, C. M. Characterization of heteropolymeric hexosaminidase A in human x mouse hybrid cells. Proc. Nat. Acad. Sci. 73: 3637-3640, 1976.
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    Konservierungsmittel
    Thimerosal (Merthiolate), Sodium azide
    Vorsichtsmaßnahmen
    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    Hexosaminidase A (HEXA)
    Andere Bezeichnung
    HEXA (HEXA Produkte)
    Hintergrund

    Synonyms: Beta-hexosaminidase subunit alpha,3.2.1.52,Beta-N-acetylhexosaminidase subunit alpha,Hexosaminidase subunit A,N-acetyl-beta-glucosaminidase subunit alpha,HEXA,

    Tissue Specificity: Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct. .

    Background: HEXA (hexosaminidase A (alpha polypeptide)) is an enzyme that in humans is encoded by the HEXA gene. Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines The HEXA gene encodes the alpha subunit of hexosaminidase A, a lysosomal enzyme involved in the breakdown of gangliosides. The HEXA gene is mapped on 15q23. Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, only the alpha subunit is able to hydrolyze GM2 gangliosides. The alpha subunit contains a key residue, Arg-424, which is essential for binding the N-acetyl-neuramanic residue of GM2 gangliosides. Chimeric constructs were expressed in HeLa cells and selected constructs were produced in the baculovirus expression system to determine their ability to degrade GM2 ganglioside in the presence of GM2 activator protein. Their results allowed them to define 2 noncontiguous sequences in the alpha subunit (amino acids 1-191 and 403-529) which, when substituted into analogous positions in the beta subunit, conferred activity against the sulfated substrate.

    Sequence Similarities: Belongs to the glycosyl hydrolase 20 family.

    Molekulargewicht
    61 kDa
    UniProt
    P06865
    Pathways
    Sensory Perception of Sound, Glycosaminoglycan Metabolic Process
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