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CACYBP Antikörper (Middle Region)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CACYBP in WB, IF, ICC, IP und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.
Produktnummer ABIN3044149

Kurzübersicht für CACYBP Antikörper (Middle Region) (ABIN3044149)

Target

Alle CACYBP Antikörper anzeigen
CACYBP (Calcyclin Binding Protein (CACYBP))

Reaktivität

  • 60
  • 11
  • 9
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 49
  • 11
Kaninchen

Klonalität

  • 51
  • 9
Polyklonal

Konjugat

  • 42
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser CACYBP Antikörper ist unkonjugiert

Applikation

  • 39
  • 18
  • 13
  • 13
  • 12
  • 12
  • 10
  • 9
  • 7
  • 6
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS)
  • Bindungsspezifität

    • 15
    • 10
    • 7
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 160-175, Middle Region

    Verwendungszweck

    Anti-SIAH Interacting Protein/CACYBP Antibody Picoband®

    Sequenz

    NTRWDYLTQV EKECKE

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-SIAH Interacting Protein/CACYBP Antibody (ABIN3044149). Tested in IP, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence in the middle region of human CACYBP, identical to the related rat and mouse sequences.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
    Immunoprecipitation, 0.5-2 μg/mL, Human
    1. Matsuzawa, S., Reed, J. C. Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses.Molec. Cell 7: 915-926, 2001. 2. Sun S, Ning X, Liu J, et al. (2007). "Overexpressed CacyBP/SIP leads to the suppression of growth in renal cell carcinoma.". Biochem. Biophys. Res. Commun. 356 (4): 864-71.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Thimerosal, 0.01 mg Sodium azide.

    Konservierungsmittel

    Thimerosal (Merthiolate), Sodium azide

    Vorsichtsmaßnahmen

    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Target

    CACYBP (Calcyclin Binding Protein (CACYBP))

    Andere Bezeichnung

    CACYBP

    Hintergrund

    Synonyms: Calcyclin-binding protein,CacyBP,hCacyBP,S100A6-binding protein,Siah-interacting protein,CACYBP,S100A6BP, SIP,PNAS-107,

    Tissue Specificity: In lung, HT-29 colonic epithelial cells, Raji B-lymphoblasts. Lesser extent in brain, heart, kidney, liver and placenta. Not expressed in CD4+ or CD8+ T-cells. Expressed in the T-cell lines HARRIS, HuT 78, Jurkat and SUP-T1, but not in the T- cell lines Peer, MOLT-4, HSB and YT. .

    Background: CACYBP (Calcyclin-binding protein), also called SIP, is a protein that in humans is encoded by the CACYBP gene. The full-length SIP cDNA encodes a predicted 228-amino acid protein. Sequence analysis of the shortest cDNA derived by 2-hybrid screening revealed an 8-amino acid difference in the deduced open reading frame followed by a stop codon, resulting in a predicted 80-amino acid protein, SIP-short (SIPS). The CACYBP gene is mapped on 1q25.1. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. The C-terminal region of SIP that is homologous to SGT1 was able to complement defects in yeast strains containing SGT1 mutant alleles, demonstrating conservation of SGT1 and SIP protein function.

    Sequence Similarities: Contains 1 CS domain.

    Molekulargewicht

    27 kDa

    Pathways

    Response to Growth Hormone Stimulus
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