NOX1 Antikörper (NADPH Oxidase 1) (AA 354-374)

Details for Product anti-NOX1 Antibody No. ABIN3044132, Anbieter: Anmelden zum Anzeigen
Antigen
  • GP91-2
  • MOX1
  • NOH-1
  • NOH1
  • NOX1a
  • NOX1alpha
  • Nox-1
  • Nox1
  • NADPH oxidase 1
  • NOX1
  • Nox1
  • nox1
Epitop
AA 354-374, Middle Region
15
14
10
10
8
7
5
4
4
3
2
2
2
2
2
1
1
1
1
1
Reaktivität
Human
101
34
31
6
4
2
2
2
2
1
1
1
1
Wirt
Kaninchen
102
10
3
Klonalität
Polyklonal
Konjugat
Dieser NOX1 Antikörper ist unkonjugiert
5
5
4
3
3
3
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
106
67
26
17
8
7
5
4
2
2
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Verwendungszweck Rabbit IgG polyclonal antibody for NADPH oxidase 1(NOX1) detection. Tested with WB, IHC-P in Human.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human NOX1(354-374aa, HIRAAGDWTENLIRAFEQQYS).
Sequenz HIRAAGDWTE NLIRAFEQQY S
Isotyp IgG
Kreuzreaktivität (Details) No cross reactivity with other proteins.
Produktmerkmale Rabbit IgG polyclonal antibody for NADPH oxidase 1(NOX1) detection. Tested with WB, IHC-P in Human.
Gene Name: NADPH oxidase 1
Protein Name: NADPH oxidase 1(NOX-1)
Reinigung Immunogen affinity purified.
Plasmids, Primers & others Plasmide, Primers & weitere NOX1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung NOX1 (NOX1 Antibody Abstract)
Hintergrund NOX1(NADPH OXIDASE 1), also known as NOH1, MOX1 or GP91-2, is an enzyme that in humans is encoded by the NOX1 gene. It is also a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. The NOX1 gene is mapped to Xq22.1. NOX1 was expressed in colon, prostate, uterus, and vascular smooth muscle, but not in peripheral blood leukocytes. The deduced 564-amino acid NOX1 protein, which is 58 % identical to CYBB, contains 6 membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. Overexpression of MOX1 in NIH 3T3 cells increased superoxide generation and cell growth. Cells expressing MOX1 had a transformed appearance, showed anchorage-independent growth, and produced tumors in athymic mice. Disruption of either Nox1 or Nox2 significantly delayed progression of motor neuron disease in these mice. However, 50 % survival rates were enhanced significantly more by Nox2 deletion than Nox1 deletion.

Synonyms: GP91 2 antibody|Mitogenic oxidase(pyridine nucleotide dependent superoxide generating) antibody|Mitogenic oxidase 1 antibody|MOX-1 antibody|MOX1 antibody|NADH/NADPH mitogenic oxidase subunit P65 MOX antibody|NADH/NADPH mitogenic oxidase subunit P65-MOX antibody|NADPH oxidase 1 antibody|NADPH oxidase 1 variant NOH 1L antibody|NADPH oxidase homolog 1 antibody|NOH 1 antibody|NOH-1 antibody|NOH1 antibody|NOX-1 antibody|Nox1 antibody|NOX1_HUMAN antibody|RP1 146H21.1 antibody
UniProt Q9Y5S8
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones, Proton Transport
Applikations-hinweise WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Kommentare

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Konzentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Konservierungs-mittel Thimerosal (Merthiolate), Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Haltbarkeit 12 months
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-NADPH Oxidase 1 (NOX1) (AA 354-374), (Middle Region) antibody (ABIN3044132) Anti-NOX1 antibody, IHC(P) IHC(P): Human Lung Cancer Tissue
Western Blotting (WB) image for anti-NADPH Oxidase 1 (NOX1) (AA 354-374), (Middle Region) antibody (ABIN3044132) Anti-NOX1 antibody, Western blottingAll lanes: Anti NOX1 at 0.5ug/ml Lane 1: HELA W...
Produkt verwendet in: Liu, Hong, Li, Ren, Wang, Xu, Shi, Xu: "A Cross Talk Between BRG1 and Males Absent on the First Contributes to Reactive Oxygen Species Production in a Mouse Model of Nonalcoholic Steatohepatitis." in: Antioxidants & redox signaling, 2018 (PubMed).

Wang, Wang, Liu, Wang, Zhao, Wang, Yin: "Cordyceps sinensis polysaccharide inhibits PDGF-BB-induced inflammation and ROS production in human mesangial cells." in: Carbohydrate polymers, Vol. 125, pp. 135-45, 2015 (PubMed).

Gu, Gong, Zhang, Dong, Zhao, Burczynski, Wang, Sun, Zhu, Han, Wang, Li: "Regulation of transforming growth factor beta 1 gene expression by dihydropteridine reductase in kidney 293T cells." in: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 91, Issue 3, pp. 187-93, 2013 (PubMed).

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