NOX1 Antikörper (Middle Region)

Produktnummer ABIN3044132

Der Kaninchen Polyklonal Anti-NOX1-Antikörper wurde für WB und IHC validiert. Er ist geeignet, NOX1 in Proben von Human zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für NOX1 Antikörper (Middle Region) (ABIN3044132)

Target: NOX1 (NADPH Oxidase 1 (NOX1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-NOX1 Antikörper

Bindungsspezifität: AA 354-374, Middle Region

Verwendungszweck: Anti-NADPH oxidase 1 NOX1 Antibody Picoband®

Sequenz: HIRAAGDWTE NLIRAFEQQY S

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-NADPH oxidase 1 NOX1 Antibody (ABIN3044132). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human NOX1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Banfi, B., Maturana, A., Jaconi, S., Arnaudeau, S., Laforge, T., Sinha, S., Ligeti, E., Demaurex, N., Krause, K.-H. A mammalian H+ channel generated through alternative splicing of the NADPH oxidase homolog NOH-1. Science 287: 138-141, 2000. 2. Behrens, M. M., Ali, S. S., Dao, D. N., Lucero, J., Shekhtman, G., Quick, K. L., Dugan, L. L. Ketamine-induced loss of phenotype of fast-spiking interneurons is mediated by NADPH-oxidase. Science 318: 1645-1647, 2007. 3. Lachgar A, Sojic N, Arbault S, et al. (1999). "Amplification of the inflammatory cellular redox state by human immunodeficiency virus type 1-immunosuppressive tat and gp160 proteins."

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-NOX1 Antikörper (ABIN3044132)

Liu, Hong, Li, Ren, Wang, Xu, Shi, Xu: "A Cross Talk Between BRG1 and Males Absent on the First Contributes to Reactive Oxygen Species Production in a Mouse Model of Nonalcoholic Steatohepatitis." in: Antioxidants & redox signaling, (2018) (PubMed).

Wang, Wang, Liu, Wang, Zhao, Wang, Yin: "Cordyceps sinensis polysaccharide inhibits PDGF-BB-induced inflammation and ROS production in human mesangial cells." in: Carbohydrate polymers, Vol. 125, pp. 135-45, (2015) (PubMed).

Gu, Gong, Zhang, Dong, Zhao, Burczynski, Wang, Sun, Zhu, Han, Wang, Li: "Regulation of transforming growth factor beta 1 gene expression by dihydropteridine reductase in kidney 293T cells." in: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 91, Issue 3, pp. 187-93, (2013) (PubMed).

Details zu NOX1

Target: NOX1 (NADPH Oxidase 1 (NOX1))

Andere Bezeichnung: NOX1

Hintergrund:

Synonyms: NADPH oxidase 1,NOX-1,1.-.-.-,Mitogenic oxidase 1,MOX-1,NADH/NADPH mitogenic oxidase subunit P65-MOX,NOH-1,NOX1,MOX1, NOH1,

Tissue Specificity: NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH- 1S is detected only in colon and colon carcinoma cells.

Background: NOX1 (NADPH OXIDASE 1), also known as NOH1, MOX1 or GP91-2, is an enzyme that in humans is encoded by the NOX1 gene. It is also a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. The NOX1 gene is mapped to Xq22.1. NOX1 was expressed in colon, prostate, uterus, and vascular smooth muscle, but not in peripheral blood leukocytes. The deduced 564-amino acid NOX1 protein, which is 58 % identical to CYBB, contains 6 membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. Overexpression of MOX1 in NIH 3T3 cells increased superoxide generation and cell growth. Cells expressing MOX1 had a transformed appearance, showed anchorage-independent growth, and produced tumors in athymic mice. Disruption of either Nox1 or Nox2 significantly delayed progression of motor neuron disease in these mice. However, 50 % survival rates were enhanced significantly more by Nox2 deletion than Nox1 deletion.

Sequence Similarities: Contains 1 FAD-binding FR-type domain.

Molekulargewicht: 65 kDa

UniProt: Q9Y5S8

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Proton Transport