KLF3 Antikörper (N-Term)

Produktnummer ABIN3044105

Produktdetails anti-KLF3 Antikörper

Target: KLF3 (Kruppel-Like Factor 3 (Basic) (KLF3))

Bindungsspezifität: AA 20-34, N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KLF3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: Anti-Krueppel-like factor 3 KLF3 Antibody Picoband®

Sequenz: SYPSNYMESM KPNKY

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Krueppel-like factor 3 KLF3 Antibody (ABIN3044105). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human KLF3, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Suske, G., Bruford, E., Philipsen, S.Mammalian SP/KLF transcription factors: bring in the family.Genomics 85: 551-556, 2005. 2. Turner, Jeremy, Nicholas Hannah, Bishop David, Matthews Jacqueline M, Crossley Merlin "The LIM protein FHL3 binds basic Krüppel-like factor/Krüppel-like factor 3 and its co-repressor C-terminal-binding protein 2". J. Biol. Chem. (United States) 278 (15): 12786-95. 2003 3. Wang MJ, Qu XH, Wang LS, et al.. "[cDNA cloning, subcellular localization and tissue expression of a new human Krüppel-like transcription factor: human basic Krüppel-like factor (hBKLF)]". Yi Chuan Xue Bao 30 (1): 1-9. 2003.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu KLF3

Target: KLF3 (Kruppel-Like Factor 3 (Basic) (KLF3))

Andere Bezeichnung: KLF3 (KLF3 Produkte)

Hintergrund:

Synonyms: Krueppel-like factor 3;Basic krueppel-like factor;CACCC-box-binding protein BKLF;TEF-2;KLF3;BKLF;

Background: KLF3 (Krueppel-like factor 3) is a protein that in humans is encoded by the KLF3 gene. It is also known as BKLF (Basic kruppel-like factor). KLF3 is a member of the Krüppel-like factors family of transcription factors. KLF3 primarily represses transcription through the transcriptional corepressor C-terminal binding protein (CtBP). Suske et al. (2005) stated that the human KLF3 gene maps to chromosome 4p14, and the mouse Klf3 gene maps to chromosome 5C3.3. Suske et al. (2005) stated that the human and mouse KLF3 proteins contain 345 and 344 amino acids, respectively. Both have a characteristic zinc finger domain, but lack the N-terminal buttonhead box found in related SP transcription factors. The repression mediated through KLF3 is also partially dependent on the sumoylation of its K10 and K197. Within KLF3's C-terminal, which is highly conserved throughout the KLF family, resides three C2H2 zinc fingers that preferentially bind the site NRG GNG NGR.

Sequence Similarities: Belongs to the krueppel C2H2-type zinc-finger protein family.

Molekulargewicht: 18 kDa

UniProt: P57682