IBSP Antikörper (C-Term)

Produktnummer ABIN3044104

Produktdetails anti-IBSP Antikörper

Target: IBSP (Integrin-Binding Sialoprotein (IBSP))

Bindungsspezifität: AA 305-324, C-Term

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IBSP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Bone sialoprotein 2(IBSP) detection. Tested with WB, IHC-P in Mouse,Rat.

Sequenz: SYYKGHGYEG YEGQNYYYHQ

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Bone sialoprotein 2(IBSP) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: integrin-binding sialoprotein
Protein Name: Bone sialoprotein 2

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bone Sialoprotein(305-324aa SYYKGHGYEGYEGQNYYYHQ), different from the related rat sequence by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Mouse, The detection limit for IBSP is approximately 2.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-IBSP Antikörper (ABIN3044104)

Zhou, Deng, Liu, Li: "Effect of kidney-reinforcing and marrow-beneficial Chinese medicine on bone metabolism-related factors following spinal cord injury in rats." in: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 485-491, (2016) (PubMed).

Cheng, Wang, Zhang, Shi: "The osteogenic potential of mesoporous bioglasses/silk and non-mesoporous bioglasses/silk scaffolds in ovariectomized rats: in vitro and in vivo evaluation." in: PLoS ONE, Vol. 8, Issue 11, pp. e81014, (2014) (PubMed).

Details zu IBSP

Target: IBSP (Integrin-Binding Sialoprotein (IBSP))

Andere Bezeichnung: IBSP (IBSP Produkte)

Hintergrund: IBSP(integrin-binding sialoprotein) is also known as BSP. The protein encoded by this gene is a major structural protein of the bone matrix. Bone sialoprotein is an acidic glycoprotein of approximately 70 kD that undergoes extensive posttranslational modifications. It constitutes approximately 12 % of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. The BSP gene is mapped to 4q22.1.

Synonyms: BNSP antibody|Bone sialoprotein 2 antibody|Bone sialoprotein II antibody|BSP antibody|BSP II antibody|BSPII antibody|Cell binding sialoprotein antibody|Cell-binding sialoprotein antibody|IBSP antibody|Integrin binding sialoprotein antibody|Integrin-binding sialoprotein antibody|SIAL_HUMAN antibody|SPII antibody

UniProt: Q61711