Glutaredoxin 2 Antikörper (Middle Region)

Produktnummer ABIN3044103

Produktdetails anti-Glutaredoxin 2 Antikörper

Target: Glutaredoxin 2 (GRX2)

Bindungsspezifität: AA 103-119, Middle Region

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Glutaredoxin 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Glutaredoxin-2, mitochondrial(GLRX2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: EYGNQFQDAL YKMTGER

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Glutaredoxin-2, mitochondrial(GLRX2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: glutaredoxin 2
Protein Name: Glutaredoxin-2, mitochondrial

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Glutaredoxin 2(103-119aa EYGNQFQDALYKMTGER), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu Glutaredoxin 2

Target: Glutaredoxin 2 (GRX2)

Andere Bezeichnung: GLRX2 (GRX2 Produkte)

Hintergrund: GLRX2(Glutaredoxin-2) also known as Glutaredoxin-2, mitochondrial or GRX2, is a protein that in humans is encoded by the GLRX2 gene. Glutaredoxins(e.g., GLRX) are a family of glutathione-dependent hydrogen donors that participate in a variety of cellular redox reactions. By sequence analysis, Lundberg et al.(2001) and Gladyshev et al.(2001) identified the GLRX2 gene within a clone mapping to chromosome 1q31.2-q31.3. Lundberg et al.(2001) determined that the GLRX2 gene contains 5 exons spanning about 9.6 kb. The GLRX2B transcript uses a first exon(exon 1B) located upstream from the first exon used by the GLRX2A transcript(exon 1A), suggesting that alternative splicing generates the isoforms. Lundberg et al.(2001)assayed reductase activity in recombinant proteins of both GLRX2 isoforms. They found that both have GSH-dependent dehydroascorbate reductase activity and 2-hydroxyethyl disulfide reductase activity.

Synonyms: bA101E13.1(GRX2 glutaredoxin(thioltransferase) 2) antibody|bA101E13.1 antibody|CGI133 antibody|Glrx2 antibody|GLRX2_HUMAN antibody|Glutaredoxin-2 antibody|Glutaredoxin-2, mitochondrial antibody|GRX2 antibody|mitochondrial antibody

Pathways: Cell RedoxHomeostasis