GATA2 Antikörper (N-Term)

Produktnummer ABIN3044102

Produktdetails anti-GATA2 Antikörper

Target: GATA2 (GATA Binding Protein 2 (GATA2))

Bindungsspezifität: AA 46-60, N-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GATA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: Rabbit IgG polyclonal antibody for Endothelial transcription factor GATA-2(GATA2) detection. Tested with WB in Human,Mouse,Rat.

Sequenz: DVFFNHLDSQ GNPYY

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Endothelial transcription factor GATA-2(GATA2) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: GATA binding protein 2
Protein Name: Endothelial transcription factor GATA-2

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human GATA2(46-60aa DVFFNHLDSQGNPYY), identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for GATA2 is approximately 5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu GATA2

Target: GATA2 (GATA Binding Protein 2 (GATA2))

Andere Bezeichnung: GATA2 (GATA2 Produkte)

Synonyme: DCML antikoerper, MONOMAC antikoerper, NFE1B antikoerper, Gata-2 antikoerper, xgata-2 antikoerper, xgata2 antikoerper, GATA-2 antikoerper, NF-E1b antikoerper, GATA2 antikoerper, Gata2 antikoerper, gata2 antikoerper, zg2 antikoerper, zgc:91840 antikoerper, GATA binding protein 2 antikoerper, GATA binding protein 2 L homeolog antikoerper, amidase antikoerper, glutamyl/aspartyl-tRNA(Gln/Asp) amidotransferasesubunit A antikoerper, hypothetical protein antikoerper, glutamyl-tRNA(Gln) amidotransferase, subunit GatA (Glu-ADT subunit A) antikoerper, GATA2 transcription factor antikoerper, putative gata binding factor antikoerper, GATA binding protein 2a antikoerper, GATA binding protein 2b antikoerper, GATA2 antikoerper, Gata2 antikoerper, gata2.L antikoerper, RR_RS20245 antikoerper, gatA2 antikoerper, Smp_018590 antikoerper, gata2a antikoerper, gata2b antikoerper

Hintergrund: GATA2(GATA binding protein 2) is a human gene which makes a protein called GATA binding protein 2 - a transcription factor. The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells. Ciciotte et al.(1997)mapped the mouse Gata2 gene to chromosome 6 by study of DNA from an interspecific backcross panel. They pointed out that the human gene had been mapped to chromosome 3 by Dorfman et al.(1992) using DNA from a panel of 12 rodent/human hybrids containing various human chromosomes and applying Southern blot analysis. By exon trapping using a PAC contig spanning a breakpoint region associated with myeloid leukemia, Wieser et al.(2000) mapped the GATA2 gene to chromosome 3q21. GATA2 is transcribed from telomere to centromere. GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells.

Synonyms: DCML antibody|Endothelial transcription factor GATA 2 antibody|Endothelial transcription factor GATA-2 antibody|Endothelial transcription factor GATA2 antibody|FLJ45948 antibody|GATA 2 antibody|GATA Binding Protein 2 antibody|GATA-binding protein 2 antibody|Gata2 antibody|GATA2_HUMAN antibody|MGC2306 antibody|MONOMAC antibody|NFE 1B antibody|NFE1B antibody|OTTHUMP00000216240 antibody

UniProt: P23769

Pathways: Stem Cell Maintenance