FER Antikörper (Middle Region)

Produktnummer ABIN3044101

Produktdetails anti-FER Antikörper

Target: FER (FER Tyrosine Kinase (FER))

Bindungsspezifität: AA 521-536, Middle Region

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FER Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Tyrosine-protein kinase Fer(FER) detection. Tested with WB, IHC-P in Human,Rat.

Sequenz: FSNIPQLIDH HYTTKQ

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Tyrosine-protein kinase Fer(FER) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: fer(fps/fes related) tyrosine kinase
Protein Name: Tyrosine-protein kinase Fer

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human FER(521-536aa FSNIPQLIDHHYTTKQ), different from the related rat and mouse sequences by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for FER is approximately 5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu FER

Target: FER (FER Tyrosine Kinase (FER))

Andere Bezeichnung: FER (FER Produkte)

Synonyme: FerT antikoerper, PPP1R74 antikoerper, TYK3 antikoerper, Fer antikoerper, Flk antikoerper, Flk_retired antikoerper, AV082135 antikoerper, C330004K01Rik antikoerper, Fert antikoerper, FER tyrosine kinase antikoerper, fer (fms/fps related) protein kinase antikoerper, FER antikoerper, Fer antikoerper

Hintergrund: FER(FPS/FES-Related tyrosine kinase) also known as TYK3, is an enzyme that in humans is encoded by the FER gene. Fer protein is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases. By in situ hybridization, Morris et al.(1990) concluded that the FER gene is located at 5q21-q22. Treatment of cells with JMP resulted in the release of FER from the cadherin complex and its accumulation in the integrin complex. The accumulation of FER in the integrin complex and the inhibitory effects of JMP could be reversed with a peptide that mimics the first coiled-coil domain of FER. The results suggested that FER mediates crosstalk between CDH2 and ITGB1. In Fer mutant mice, leukocyte emigration was exaggerated in response to LPS without altering vascular permeability, suggesting that FER has a role in regulating innate immunity.

Synonyms: c FER antibody|Fer(fps/fes related) tyrosine kinase(phosphoprotein NCP94) antibody|Fer(fps/fes related) tyrosine kinase antibody|FER antibody|FER_HUMAN antibody|p94 FER antibody|p94-FER antibody|Phosphoprotein NCP94 antibody|Proto oncogene tyrosine protein kinase FER antibody|Proto-oncogene c-Fer antibody|TYK3 antibody|Tyrosine kinase 3 antibody|Tyrosine-protein kinase Fer antibody

UniProt: P16591