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FER Antikörper (Middle Region)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch FER in WB und IHC. Er zeigt eine Reaktivität gegenüber Human und Ratte.
Produktnummer ABIN3044101

Kurzübersicht für FER Antikörper (Middle Region) (ABIN3044101)

Target

Alle FER Antikörper anzeigen
FER (FER Tyrosine Kinase (FER))

Reaktivität

  • 70
  • 61
  • 16
  • 6
  • 4
  • 4
  • 2
  • 1
  • 1
Human, Ratte

Wirt

  • 83
  • 10
Kaninchen

Klonalität

  • 83
  • 10
Polyklonal

Konjugat

  • 45
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Dieser FER Antikörper ist unkonjugiert

Applikation

  • 78
  • 33
  • 26
  • 26
  • 14
  • 13
  • 12
  • 6
  • 4
  • 4
  • 4
  • 2
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC)
  • Bindungsspezifität

    • 26
    • 15
    • 10
    • 8
    • 6
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 521-536, Middle Region

    Verwendungszweck

    Anti-Tyrosine-protein kinase Fer FER Antibody Picoband®

    Sequenz

    FSNIPQLIDH HYTTKQ

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-Tyrosine-protein kinase Fer FER Antibody (ABIN3044101). Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence in the middle region of human FER, different from the related rat and mouse sequences by two amino acids.

    Isotyp

    IgG
  • Applikationshinweise

    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
    Western blot, 0.1-0.5 μg/mL, Human, Rat
    1. Arregui, C., Pathre, P., Lilien, J., Balsamo, J. The nonreceptor tyrosine kinase Fer mediates cross-talk between N-cadherin and beta-1-integrins. J. Cell Biol. 149: 1263-1273, 2000. 2. McCafferty, D.-M., Craig, A. W. B., Senis, Y. A., Greer, P. A. Absence of Fer protein-tyrosine kinase exacerbates leukocyte recruitment in response to endotoxin. J. Immun. 168: 4930-4935, 2002. 3. Morris, C., Heisterkamp, N., Hao, Q. L., Testa, J. R., Groffen, J. The human tyrosine kinase gene (FER) maps to chromosome 5 and is deleted in myeloid leukemias with a del(5q). Cytogenet. Cell Genet. 53: 196-200, 1990.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

    Konservierungsmittel

    Thimerosal (Merthiolate), Sodium azide

    Vorsichtsmaßnahmen

    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Target

    FER (FER Tyrosine Kinase (FER))

    Andere Bezeichnung

    FER

    Hintergrund

    Synonyms: Tyrosine-protein kinase Fer,2.7.10.2,Feline encephalitis virus-related kinase FER,Fujinami poultry sarcoma/Feline sarcoma-related protein Fer,Proto-oncogene c-Fer,Tyrosine kinase 3,p94-Fer,FER,TYK3,

    Tissue Specificity: Isoform 1 is detected in normal colon and in fibroblasts (at protein level). Isoform 3 is detected in normal testis, in colon carcinoma-derived metastases in lung, liver and ovary, and in colon carcinoma and hepato carcinoma cell lines (at protein level). Isoform 3 is not detected in normal colon or in normal fibroblasts (at protein level). Widely expressed. .

    Background: FER (FPS/FES-Related tyrosine kinase) also known as TYK3, is an enzyme that in humans is encoded by the FER gene. Fer protein is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases. By in situ hybridization, Morris et al. (1990) concluded that the FER gene is located at 5q21-q22. Treatment of cells with JMP resulted in the release of FER from the cadherin complex and its accumulation in the integrin complex. The accumulation of FER in the integrin complex and the inhibitory effects of JMP could be reversed with a peptide that mimics the first coiled-coil domain of FER. The results suggested that FER mediates crosstalk between CDH2 and ITGB1. In Fer mutant mice, leukocyte emigration was exaggerated in response to LPS without altering vascular permeability, suggesting that FER has a role in regulating innate immunity.

    Sequence Similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.

    Molekulargewicht

    95 kDa

    UniProt

    P16591
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