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Splicing Factor 1 Antikörper (N-Term)

Dieses Anti-Splicing Factor 1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Splicing Factor 1 in WB, IHC, IF, ICC, IHC (fro) und FACS. Geeignet für Human, Maus und Ratte.
Produktnummer ABIN3044091

Kurzübersicht für Splicing Factor 1 Antikörper (N-Term) (ABIN3044091)

Target

Alle Splicing Factor 1 (SF1) Antikörper anzeigen
Splicing Factor 1 (SF1)

Reaktivität

  • 59
  • 50
  • 18
  • 12
  • 10
  • 10
  • 8
  • 7
  • 7
  • 6
  • 5
  • 5
  • 3
  • 2
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 69
  • 5
Kaninchen

Klonalität

  • 70
  • 4
Polyklonal

Konjugat

  • 48
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Splicing Factor 1 Antikörper ist unkonjugiert

Applikation

  • 64
  • 30
  • 24
  • 16
  • 13
  • 13
  • 13
  • 10
  • 8
  • 5
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
  • Bindungsspezifität

    • 29
    • 9
    • 7
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 11-30, N-Term

    Verwendungszweck

    Anti-splicing factor 1/SF1 Antibody Picoband®

    Sequenz

    DFPSKKRKRS RWNQDTMEQK

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-splicing factor 1/SF1 Antibody (ABIN3044091). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the N-terminus of human splicing factor 1, identical to the related rat and mouse sequences.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat
    Immunocytochemistry , 0.5-1 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    1. Toda, T., Iida, A., Miwa, T., Nakamura, Y., Imai, T. Isolation and characterization of a novel gene encoding nuclear protein at a locus (D11S636) tightly linked to multiple endocrine neoplasia type 1 (MEN1). Hum. Molec. Genet. 3: 465-470, 1994. 2. Caslini, C., Spinelli, O., Cazzaniga, G., Golay, J., De Gioia, L., Pedretti, A., Breviario, F., Amaru, R., Barbui, T., Biondi, A., Introna, M., Rambaldi, A. Identification of two novel isoforms of the ZNF162 gene: a growing family of signal transduction and activator of RNA proteins. Genomics 42: 268-277, 1997.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

    Konservierungsmittel

    Thimerosal (Merthiolate), Sodium azide

    Vorsichtsmaßnahmen

    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Target

    Splicing Factor 1 (SF1)

    Andere Bezeichnung

    SF1

    Hintergrund

    Synonyms: Splicing factor 1,Mammalian branch point-binding protein,BBP,mBBP,Transcription factor ZFM1,Zinc finger gene in MEN1 locus,Zinc finger protein 162,SF1,ZFM1, ZNF162,

    Tissue Specificity: Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart. .

    Background: Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene. This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

    Sequence Similarities: Belongs to the BBP/SF1 family.

    Molekulargewicht

    68-80 kDa

    UniProt

    Q15637

    Pathways

    Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location
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