p63 Antikörper (C-Term)

Produktnummer ABIN3044067

Dieses Anti-p63-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von p63 in WB, IHC, ICC, IF und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für p63 Antikörper (C-Term) (ABIN3044067)

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser p63 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-p63 Antikörper

Bindungsspezifität: AA 664-680, C-Term

Verwendungszweck: Anti-p63/TP63 Antibody Picoband®

Sequenz: DMDARRNKQQ RIKEEGE

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-p63/TP63 Antibody (ABIN3044067). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human p63, different from the related rat and mouse sequences by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry , 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Hibi, K., Trink, B., Patturajan, M., Westra, W. H., Caballero, O. L., Hill, D. E., Ratovitski, E. A., Jen, J., Sidransky, D. AIS is an oncogene amplified in squamous cell carcinoma. Proc. Nat. Acad. Sci. 97: 5462-5467, 2000. 2. Osada, M., Ohba, M., Kawahara, C., Ishioka, C., Kanamaru, R., Katoh, I., Ikawa, Y., Nimura, Y., Nakagawara, A., Obinata, M., Ikawa, S. Cloning and functional analysis of human p51, which structurally and functionally resembles p53. Nature Med. 4: 839-843, 1998. 3. Senoo, M., Seki, N., Ohira, M., Sugano, S., Watanabe, M., Inuzuka, S., Okamoto, T., Tachibana, M., Tanaka, T., Shinkai, Y., Kato, H. A second p53-related protein, p73L, with high homology to p73. Biochem. Biophys. Res. Commun. 248: 603-607, 1998. Note: Erratum: Biochem. Biophys. Res. Commun. 250: 536 only, 1998. 4. Trink, B., Okami, K., Wu, L., Sriuranpong, V., Jen, J., Sidransky, D. A new human p53 homologue. (Letter) Nature Med. 4: 747-748, 1998. 5. Yang, A., Kaghad, M., Wang, Y., Gillett, E., Fleming, M. D., Dotsch, V., Andrews, N. C., Caput, D., McKeon, F. p63, a p53 homolog at 3q27-29, encodes multiple products with transactivating, death-inducing, and dominant-negative activities. Molec. Cell 2: 305-316, 1998.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu p63

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Andere Bezeichnung: TP63

Hintergrund:

Synonyms: Tumor protein 63,p63,Chronic ulcerative stomatitis protein,CUSP,Keratinocyte transcription factor KET,Transformation-related protein 63,TP63,Tumor protein p73-like,p73L,p40,p51,TP63,KET, P63, P73H, P73L, TP73L,

Tissue Specificity: Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues. .

Background: TP63 (Tumor Protein p63), also known as KET, is a protein that in humans is encoded by the TP63 gene. Yang et al. (1998) described the cloning of tumor protein p63, which shows strong homology to the tumor suppressor p53 and the p53-related protein p73. By fluorescence in situ hybridization, Yang et al. (1998) localized the human TP63 gene to chromosome 3q27-q29. Hibi et al. (2000) stated that p53 homologs known variously as p40, p51, p63, and p73L (Trink et al., 1998, Yang et al., 1998, Osada et al., 1998, Senoo et al., 1998) are isoforms of the same gene, which Hibi et al. (2000) referred to as AIS for amplified in squamous cell carcinoma.

Sequence Similarities: Belongs to the p53 family.

Molekulargewicht: 75 kDa

UniProt: Q9H3D4