TJP3 Antikörper (C-Term)

Produktnummer ABIN3044037

Dieses Anti-TJP3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TJP3 in WB und IHC. Geeignet für Ratte und Maus.

Kurzübersicht für TJP3 Antikörper (C-Term) (ABIN3044037)

Target: TJP3 (Tight Junction Protein 3 (Zona Occludens 3) (TJP3))

Reaktivität: Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TJP3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-TJP3 Antikörper

Bindungsspezifität: AA 885-905, C-Term

Verwendungszweck: Anti-Zonula occludens protein 3/TJP3 Antibody Picoband®

Sequenz: RARDVESSDD EGYDWGPATD L

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Zonula occludens protein 3/TJP3 Antibody (ABIN3044037). Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Zonula occludens protein 3, identical to the related rat sequence.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Rat, Mouse
1. Haskins, J., Gu, L., Wittchen, E. S., Hibbard, J., Stevenson, B. R. ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin. J. Cell Biol. 141: 199-208, 1998. 2. Itoh, M., Furuse, M., Morita, K., Kubota, K., Saitou, M., Tsukita, S. binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins. J. Cell Biol. 147: 1351-1363, 1999. 3. Roh, M. H., Liu, C.-J., Laurinec, S., Margolis, B. The carboxyl terminus of zona occludens-3 binds and recruits a mammalian homologue of discs lost to tight junctions. J. Biol. Chem. 277: 27501-27509, 2002.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu TJP3

Target: TJP3 (Tight Junction Protein 3 (Zona Occludens 3) (TJP3))

Andere Bezeichnung: TJP3

Hintergrund:

Synonyms: Tight junction protein ZO-3,Tight junction protein 3,Zona occludens protein 3,Zonula occludens protein 3,Tjp3,Zo3,

Background: TJP3 (TIGHT JUNCTION PROTEIN 3), also called ZO3, is a protein that in humans is encoded by the TJP3 gene. TJP3 is a member of the family of membrane-associated guanylate kinase-like proteins (MAGUK) that associate with intracellular junctions (Itoh et al., 1999). Haskins et al. (1998) showed that recombinant Tjp3 binds in vitro translated Tjp1 and binds ly to the cytoplasmic tail of occludin but no binding was detected between Tjp3 and Tjp2. Roh et al. (2002) determined that Tjp3 interacted with PATJ (INADL) and that both proteins colocalized to tight junctions in vivo. In vitro studies showed that the sixth and eighth PDZ domains of PATJ mediated interaction with the Tjp3 C-terminal domain and with Cldn1, respectively.

Sequence Similarities: Belongs to the MAGUK family.

Molekulargewicht: 150 kDa