CUEDC2 Antikörper (C-Term)

Produktnummer ABIN3044031

Produktdetails anti-CUEDC2 Antikörper

Target: CUEDC2 (CUE Domain Containing 2 (CUEDC2))

Bindungsspezifität: AA 267-287, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CUEDC2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Verwendungszweck: Anti-CUEDC2 Antibody Picoband®

Sequenz: EAEEMKATYI NLKPARKYRF H

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-CUEDC2 Antibody (ABIN3044031). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CUEDC2, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Hartz, P. A. Personal Communication. Baltimore, Md. 7/26/2011. 2. Pan, X., Zhou, T., Tai, Y.-H., Wang, C., Zhao, J., Cao, Y., Chen, Y., Zhang, P.-J., Yu, M., Zhen, C., Mu, R., Bai, Z.-F., and 10 others. Elevated expression of CUEDC2 protein confers endocrine resistance in breast cancer. Nature Med. 17: 708-714, 2011. 3. Zhang, P.-J., Zhao, J., Li, H.-Y., Man, J.-H., He, K., Zhou, T., Pan, X., Li, A.-L., Gong, W.-L., Jin, B.-F., Xia, Q., Yu, M., Shen, B.-F., Zhang, X.-M. CUE domain containing 2 regulates degradation of progesterone receptor by ubiquitin-proteasome. EMBO J. 26: 1831-1842, 2007.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu CUEDC2

Target: CUEDC2 (CUE Domain Containing 2 (CUEDC2))

Andere Bezeichnung: CUEDC2 (CUEDC2 Produkte)

Hintergrund:

Synonyms: CUE domain-containing protein 2,CUEDC2,C10orf66,HOYS6,

Tissue Specificity: Significantly up-regulated in breast tumor tissues compared with matched adjacent normal tissues (at protein level). Levels inversely correlate with ESR1 in breast cancers and are lower in low-grade tumors compared to high-grade tumors.

Background: CUEDC2 (Cue Domain-Containing Protein 2) is involved in ubiquitin-and proteasome-mediated degradation of progesterone receptor (PR, or PGR) and estrogen receptor (ER)-alpha. Hartz (2011) mapped the CUEDC2 gene to chromosome 10q24.32 based on an alignment of the CUEDC2 sequence with the genomic sequence (GRCh37). Using coimmunoprecipitation analysis and protein pull-down assays, Zhang et al. (2007) confirmed that CUEDC2 interacted with the PRB isoform of PR. Mutation analysis revealed that the IF domain of PRB and the CUE domain of CUEDC2 were required for the interaction. CUEDC2 reduced PRB protein content and promoted progesterone-induced PRB degradation via the ubiquitin-proteasome pathway.

Sequence Similarities: Belongs to the CUEDC2 family.

Molekulargewicht: 32 kDa, 19 kDa

UniProt: Q9H467