ZEB2 Antikörper (C-Term)

Produktnummer ABIN3044028

Dieses Anti-ZEB2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ZEB2 in WB und IHC. Geeignet für Human, Maus und Ratte.

Kurzübersicht für ZEB2 Antikörper (C-Term) (ABIN3044028)

Target: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ZEB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-ZEB2 Antikörper

Bindungsspezifität: AA 948-962, C-Term

Verwendungszweck: Anti-Smad Interacting Protein 1/ZEB2 Antibody Picoband®

Sequenz: DMQQRRKYQR KQGFQ

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Smad Interacting Protein 1/ZEB2 Antibody (ABIN3044028). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ZEB2, identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
1. Beltran, M., Puig, I., Pena, C., Garcia, J. M., Alvarez, A. B., Pena, R., Bonilla, F., Garcia de Herreros, A. A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial-mesenchymal transition. Genes Dev. 22: 756-769, 2008. 2. Nagase, T., Ishikawa, K., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 5: 31-39, 1998. 3. Vandewalle, C., Comijn, J., De Craene, B., Vermassen, P., Bruyneel, E., Andersen, H., Tulchinsky, E., Van Roy, F., Berx, G. SIP1/ZEB2 induces EMT by repressing genes of different epithelial cell-cell junctions. Nucleic Acids Res. 33: 6566-6578, 2005. 4. Wakamatsu, N., Yamada, Y., Yamada, K., Ono, T., Nomura, N., Taniguchi, H., Kitoh, H., Mutoh, N., Yamanaka, T., Mushiake, K., Kato, K., Sonta, S., Nagaya, M. Mutations in SIP1, encoding Smad interacting protein-1, cause a form of Hirschsprung disease. Nature Genet. 27: 369-370, 2001.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.01 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu ZEB2

Target: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Andere Bezeichnung: ZEB2

Hintergrund:

Synonyms: Zinc finger E-box-binding homeobox 2,Smad-interacting protein 1,SMADIP1,Zinc finger homeobox protein 1b,ZEB2,KIAA0569, SIP1, ZFHX1B, ZFX1B,HRIHFB2411,

Tissue Specificity: Highly expressed in spleen, lymph node, thymus, tonsil and at lower levels in lung. Highly expressed in promyelocytic HL-60 cells and in B-cell lines.

Background: ZEB2 (Zinc finger E-box-binding homeobox2), also known as SIP1or ZINC FINGER HOMEOBOX 1B (ZFHX1B), is a protein that in humans is encoded by the ZEB2 gene. The ZEB2 gene is a member of the ZEB1/Drosophila Zfh1 family of 2-handed zinc finger/homeodomain proteins and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs, the transducers of TGF-beta signaling, and interacts with the nucleosome remodeling and histone deacetylation (NURD) complex. By radiation hybrid analysis, Nagase et al. (1998) mapped the ZEB2 gene to chromosome 2. Wakamatsu et al. (2001) mapped the ZEB2 gene to chromosome 2q22. Vandewalle et al. (2005) showed that expression of mouse Sip1 in human epithelial cells caused a morphologic change from an epithelial to a mesenchymal phenotype. Expression of SNAI1 in epithelial cells triggers an epithelial-mesenchyme transition. Beltran et al. (2008) showed that synthesis of ZEB2 was upregulated following SNAI1 expression in human cell lines.

Sequence Similarities: Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.

Molekulargewicht: 180 kDa

UniProt: O60315

Pathways: Tube Formation