Tyrosine Hydroxylase Antikörper (Middle Region)

Produktnummer ABIN3043947

Dieses Anti-Tyrosine Hydroxylase-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Tyrosine Hydroxylase in WB, IHC und IF. Geeignet für Ratte und Maus. Dieses Primary Antibody wurde in 10+ Publikationen zitiert.

Kurzübersicht für Tyrosine Hydroxylase Antikörper (Middle Region) (ABIN3043947)

Target: Tyrosine Hydroxylase (TH)

Reaktivität: Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tyrosine Hydroxylase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-Tyrosine Hydroxylase Antikörper

Bindungsspezifität: AA 193-222, Middle Region

Verwendungszweck: Anti-Tyrosine Hydroxylase/TH Antibody Picoband®

Sequenz: KVPWFPRKVS ELDKCHHLVT KFDPDLDLDH

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Rabbit IgG polyclonal antibody for Tyrosine 3-monooxygenase(TH) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: tyrosine hydroxylase
Protein Name: Tyrosine 3-monooxygenase

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Tyrosine Hydroxylase, identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Immunofluorescence, 5 μg/mL, Mouse, Rat
1. Haavik J, Toska K (June 1998). "Tyrosine hydroxylase and Parkinson's disease". Mol. Neurobiol. 16 (3): 285-309. 2. Kaufman S (1995). "Tyrosine hydroxylase". Adv. Enzymol. Relat. Areas Mol. Biol. Advances in Enzymology - and Related Areas of Molecular Biology 70: 103-220. Nagatsu T (1995). "Tyrosine hydroxylase: human isoforms, structure and regulation in physiology and pathology". Essays Biochem.30: 15-35.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Tyrosine Hydroxylase Antikörper (ABIN3043947)

Hu, Wang, Ren, Liu: "Autonomic remodeling may be responsible for decreased incidence of aortic dissection in STZ-induced diabetic rats via down-regulation of matrix metalloprotease 2." in: BMC cardiovascular disorders, Vol. 16, Issue 1, pp. 200, (2017) (PubMed).

Su, Fu, Zhang, Shi, Zhang, Gao: "Identification and expression of SRF targeted by miR-133a during early development of Paralichthys olivaceus." in: Fish physiology and biochemistry, Vol. 41, Issue 5, pp. 1093-104, (2015) (PubMed).

Deng, Jiao, Mi, Xu, Li, Wang, Liu, Xu: "Melatonin inhibits manganese-induced motor dysfunction and neuronal loss in mice: involvement of oxidative stress and dopaminergic neurodegeneration." in: Molecular neurobiology, Vol. 51, Issue 1, pp. 68-88, (2015) (PubMed).

Li, Jiang, Jiang, Yang, Ma, Yang: "Effect of Guizhi Decoction ([symbols; see text]) on heart rate variability and regulation of cardiac autonomic nervous imbalance in diabetes mellitus rats." in: Chinese journal of integrative medicine, Vol. 20, Issue 7, pp. 524-33, (2014) (PubMed).

Zhao, Cheng, Fan, Yang, Ye, Cui, Wei, Lao, Cai, Han, Rong et al.: "Botanical drug puerarin coordinates with nerve growth factor in the regulation of neuronal survival and neuritogenesis via activating ERK1/2 and PI3K/Akt signaling pathways in the neurite extension ..." in: CNS neuroscience & therapeutics, Vol. 21, Issue 1, pp. 61-70, (2014) (PubMed).

Huang, Zhu, Zhang, Zhu, Liu, Zhu, Wang, Li, Yang, Dong, Liu, Chen, Zhang, Yang, Deng, Fan, Wang, Liu, Ma, Fu, Wu: "S100+ cells: a new neuro-immune cross-talkers in lymph organs." in: Scientific reports, Vol. 3, pp. 1114, (2013) (PubMed).

Hu, Ye, Wang, Zhang, Chen, Guo: "Bone morphogenetic proteins induce rabbit bone marrow-derived mesenchyme stem cells to differentiate into osteoblasts via BMP signals pathway." in: Artificial cells, nanomedicine, and biotechnology, Vol. 41, Issue 4, pp. 249-54, (2013) (PubMed).

Wan, Liu, Liu, Cai, Chen: "Effect of adenovirus-mediated brain derived neurotrophic factor in early retinal neuropathy of diabetes in rats." in: International journal of ophthalmology, Vol. 3, Issue 2, pp. 145-8, (2012) (PubMed).

Wu, Ge, Zeng, Zhang: "Induced multilineage differentiation of chicken embryonic germ cells via embryoid body formation." in: Stem cells and development, Vol. 19, Issue 2, pp. 195-202, (2010) (PubMed).

Wu, Zhao, Zhu, Peng, Jia, Wu, Zheng, Wu: "A novel function of microRNA let-7d in regulation of galectin-3 expression in attention deficit hyperactivity disorder rat brain." in: Brain pathology (Zurich, Switzerland), Vol. 20, Issue 6, pp. 1042-54, (2010) (PubMed).

Details zu Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Andere Bezeichnung: Tyrosine Hydroxylase

Hintergrund:

Synonyms: Tyrosine 3-monooxygenase,1.14.16.2,Tyrosine 3-hydroxylase,TH,TH,TYH,

Tissue Specificity: Mainly expressed in the brain and adrenal glands.

Background: TH is equal to tyrosine hydroxylase. The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. In humans, tyrosine hydroxylase is encoded by the TH gene, and the enzyme is present in the central nervous system (CNS), peripheral sympathetic neurons and the adrenal medulla. Tyrosine hydroxylase, phenylalanine hydroxylase and tryptophan hydroxylase together make up the family of aromatic amino acid hydroxylases (AAAHs).

Sequence Similarities: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

Molekulargewicht: 59 kDa

Gen-ID: 7054

UniProt: P07101

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour