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LCK Antikörper (C-Term)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch LCK in WB, IHC, IF, FACS, ICC und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.
Produktnummer ABIN3043870

Kurzübersicht für LCK Antikörper (C-Term) (ABIN3043870)

Target

Alle LCK Antikörper anzeigen
LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Reaktivität

  • 161
  • 92
  • 55
  • 7
  • 6
  • 5
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 1
Human, Maus, Ratte

Wirt

  • 138
  • 22
  • 2
Kaninchen

Klonalität

  • 137
  • 26
Polyklonal

Konjugat

  • 99
  • 7
  • 7
  • 5
  • 5
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Dieser LCK Antikörper ist unkonjugiert

Applikation

  • 120
  • 79
  • 26
  • 26
  • 25
  • 20
  • 14
  • 13
  • 13
  • 10
  • 6
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Bindungsspezifität

    • 33
    • 16
    • 10
    • 9
    • 9
    • 8
    • 8
    • 6
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 468-506, C-Term

    Verwendungszweck

    Anti-Lck Antibody Picoband®

    Sequenz

    ELYQLMRLCW KERPEDRPTF DYLRSVLEDF FTATEGQYQ

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins.

    Produktmerkmale

    Anti-Lck Antibody Picoband® (ABIN3043870). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the C-terminus of human Lck, different from the related mouse and rat sequences by three amino acids.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Mouse
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Anderson, S. J., Levin, S. D., Perlmutter, R. M. Protein tyrosine kinase p56(lck) controls allelic exclusion of T-cell receptor beta-chain genes. Nature 365: 552-554, 1993. 2. Burnett, R. C., David, J.-C., Harden, A. M., Le Beau, M. M., Rowley, J. D., Diaz, M. O. The LCK gene is involved in the t(1,7)(p34,q34) in the T-cell acute lymphoblastic leukemia derived cell line, HSB-2. Genes Chromosomes Cancer 3: 461-467, 1991. 3. Marth, J. D., Disteche, C., Pravtcheva, D., Ruddle, F., Krebs, E. G., Perlmutter, R. M. Localization of a lymphocyte-specific protein tyrosine kinase gene (lck) at a site of frequent chromosomal abnormalities in human lymphomas. Proc. Nat. Acad. Sci. 83: 7400-7404, 1986.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

    Andere Bezeichnung

    LCK

    Hintergrund

    Synonyms: Tyrosine-protein kinase Lck,2.7.10.2,Leukocyte C-terminal Src kinase,LSK,Lymphocyte cell-specific protein-tyrosine kinase,Protein YT16,Proto-oncogene Lck,T cell-specific protein-tyrosine kinase,p56-LCK,LCK,

    Tissue Specificity: Expressed specifically in lymphoid cells.

    Background: Lck (or lymphocyte-specific protein tyrosine kinase) is a 56 kDa protein that is found inside specializedcells of the immune system called lymphocytes. The human LCK gene is mapped to chromosome 1p35-p32. This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described.

    Molekulargewicht

    58 kDa

    Gen-ID

    3932

    UniProt

    P06239

    Pathways

    T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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