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HSPA2 Antikörper (C-Term)

Der Kaninchen Polyklonal Anti-HSPA2-Antikörper wurde für WB, IHC, ICC, IF, FACS und IHC (fro) validiert. Er ist geeignet, HSPA2 in Proben von Human, Ratte und Maus zu detektieren.
Produktnummer ABIN3043850

Kurzübersicht für HSPA2 Antikörper (C-Term) (ABIN3043850)

Target

Alle HSPA2 Antikörper anzeigen
HSPA2 (Heat Shock 70kDa Protein 2 (HSPA2))

Reaktivität

  • 53
  • 31
  • 17
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human, Ratte, Maus

Wirt

  • 48
  • 19
Kaninchen

Klonalität

  • 39
  • 28
Polyklonal

Konjugat

  • 39
  • 11
  • 5
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Dieser HSPA2 Antikörper ist unkonjugiert

Applikation

  • 53
  • 27
  • 18
  • 17
  • 11
  • 9
  • 4
  • 2
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Bindungsspezifität

    • 9
    • 8
    • 7
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 564-598, C-Term

    Verwendungszweck

    Anti-HSPA2 Antibody Picoband®

    Sequenz

    KISEQDKNKI LDKCQEVINW LDRNQMAEKD EYEHK

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-HSPA2 Antibody Picoband® (ABIN3043850). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the C-terminus of human HSPA2, identical to the related mouse and rat sequences.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Bonnycastle, L. L. C., Yu, C.-E., Hunt, C. R., Trask, B. J., Clancy, K. P., Weber, J. L., Patterson, D., Schellenberg, G. D. Cloning, sequencing, and mapping of the human chromosome 14 heat shock protein gene (HSPA2). Genomics 23: 85-93, 1994. 2. Dix, D. J., Allen, J. W., Collins, B. W., Mori, C., Nakamura, N., Poorman-Allen, P., Goulding, E. H., Eddy, E. M. Targeted gene disruption of Hsp70-2 results in failed meiosis, germ cell apoptosis, and male infertility. Proc. Nat. Acad. Sci. 93: 3264-3268, 1996. 3. Hunt, C. R., Gasser, D. L., Chaplin, D. D., Pierce, J. C., Kozak, C. A. Chromosomal localization of five murine HSP70 gene family members: Hsp70-1, Hsp70-2, Hsp70-3, Hsc70t and Grp78. Genomics 16: 193-198, 1993.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    HSPA2 (Heat Shock 70kDa Protein 2 (HSPA2))

    Andere Bezeichnung

    HSPA2

    Hintergrund

    Synonyms: Heat shock-related 70 kDa protein 2,Heat shock 70 kDa protein 2,HSPA2,

    Tissue Specificity: Expressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues. .

    Background: HSPA2 (heat shock 70 kDa protein 2) is also known as HEAT-SHOCK PROTEIN, 70-KD, 2, HSP70-2, HEAT-SHOCK PROTEIN, 70-KD, 3 or HSP70-3. Analysis of the sequence indicated that HSPA2 is the human homolog of the murine Hsp70-2 gene, with 91.7 % identity in the nucleotide coding sequence and 98.2 % in the corresponding amino acid sequence. HSPA2 has less amino acid homology to the other members of the human HSP70 gene family. HSPA2 is constitutively expressed in most tissues, with very high levels in testis and skeletal muscle. The HSPA2 gene is located on chromosome 14q22-q24. Immunohistochemical analysis detected weak expression of HSPA2 in spermatocytes and stronger expression in spermatids and in the tail of mature sperm. HSPA2 may be critical to sperm maturation through its role as a protein chaperone.

    Sequence Similarities: Contains 1 HMG box DNA-binding domain.

    Molekulargewicht

    70 kDa

    Gen-ID

    3306

    UniProt

    P54652
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