HSP70 1A Antikörper (C-Term)

Produktnummer ABIN3043849

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch HSP70 1A in WB, IHC, IF, ICC und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 13+ Publikationen erwähnt.

Kurzübersicht für HSP70 1A Antikörper (C-Term) (ABIN3043849)

Target: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSP70 1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-HSP70 1A Antikörper

Bindungsspezifität: AA 559-596, C-Term

Verwendungszweck: Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband®

Sequenz: KGKISEADKK KVLDKCQEVI SWLDANTLAE KDEFEHKR

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband® (ABIN3043849). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp70, different from the related mouse sequence by five amino acids, and from the related rat sequence by three amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Becker, T., Hartl, F.-U., Wieland, F. CD40, an extracellular receptor for binding and uptake of Hsp70-peptide complexes. J. Cell Biol. 158: 1277-1285, 2002. 2. Gehrig, S. M., van der Poel, C., Sayer, T. A., Schertzer, J. D., Henstridge, D. C., Church, J. E., Lamon, S., Russell, A. P., Davies, K. E., Febbraio, M. A., Lynch, G. S. Hsp72 preserves muscle function and slows progression of severe muscular dystrophy. Nature 484: 394-398, 2012. 3. Shimizu, S., Nomura, K., Ujihara, M., Demura, H. An additional exon of stress-inducible heat shock protein 70 gene (HSP70-1). Biochem. Biophys. Res. Commun. 257: 193-198, 1999.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-HSP70 1A Antikörper (ABIN3043849)

Zeng, Xiao, Deng, Li: "Chondroprotective Effects and Multitarget Mechanisms of Fu Yuan Capsule in a Rat Osteoarthritis Model." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 8985623, (2017) (PubMed).

Zhang, Tang, Li, Li, Zhang, Yan, Cui: "Effects of intracerebral hemorrhage and subsequent minimally invasive hematoma aspiration on expression of apoptosisrelated genes in rats." in: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 5371-8, (2016) (PubMed).

Chen, Wan, Huang, Wang, Liu, Wang: "Study on the immune response to recombinant Hsp70 protein from Megalobrama amblycephala." in: Immunobiology, Vol. 219, Issue 11, pp. 850-8, (2014) (PubMed).

Jing, Liu, Chen, Hu, Xiao, Ma: "Acute effect of copper and cadmium exposure on the expression of heat shock protein 70 in the Cyprinidae fish Tanichthys albonubes." in: Chemosphere, Vol. 91, Issue 8, pp. 1113-22, (2013) (PubMed).

Pu, Shi, Wang, Wang, Guo, Liu, Sun, Gao, Zhou: "Effects of minocycline on the expression of NGF and HSP70 and its neuroprotection role following intracerebral hemorrhage in rats." in: Journal of biomedical research, Vol. 25, Issue 4, pp. 292-8, (2013) (PubMed).

Chen, Zuo, Yang, Lu, Wang, Wang, Tu, Chen, Liu, Liu, Xiao: "Severe multiple organ injury in HSF1 knockout mice induced by lipopolysaccharide is associated with an increase in neutrophil infiltration and surface expression of adhesion molecules." in: Journal of leukocyte biology, Vol. 92, Issue 4, pp. 851-7, (2012) (PubMed).

Li, Roubeix, Wang, Shi, Liu, Baudouin, Chen: "Therapeutic efficacy of trehalose eye drops for treatment of murine dry eye induced by an intelligently controlled environmental system." in: Molecular vision, Vol. 18, pp. 317-29, (2012) (PubMed).

Fan, Jiang, Yang, Liu, Song, Pan: "Effects of adrenergic agents on stress-induced brain microstructural and immunochemical changes in adult male Wistar rats." in: Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft, Vol. 193, Issue 5, pp. 418-24, (2011) (PubMed).

Xu, Luo, Wu, Chen, Xu, Gao, He, Geng, Xiao, Zhang, Chen, Li: "Immunohistological evaluation of skin responses after treatment using a fractional ultrapulse carbon dioxide laser on back skin." in: Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.], Vol. 37, Issue 8, pp. 1141-9, (2011) (PubMed).

Ouyang, Gao, Wang, Xie, Lei, Zhou, Liao, Zhong, Tang: "Thermoseed hyperthermia treatment of mammary orthotopic transplantation tumors in rats and impact on immune function." in: Oncology reports, Vol. 24, Issue 4, pp. 973-9, (2010) (PubMed).

Yi, Peng, Chang, Peng, Yan, Lin: "Effect of pre-moxibustion on apoptosis and proliferation of gastric mucosa cells." in: World journal of gastroenterology, Vol. 13, Issue 15, pp. 2174-8, (2007) (PubMed).

Wu, Cao, Gao, Chen, Wang, Zumbika, Qian: "Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells." in: World journal of gastroenterology, Vol. 10, Issue 20, pp. 2944-8, (2004) (PubMed).

Gnatyshak, Loginskiĭ, Mazurok: "[Homo- and autotransplantation of bone marrow in radiation and chemotherapy of oncologic patients]." in: Vrachebnoe delo, Vol. 4, pp. 129-32, (1973) (PubMed).

Details zu HSP70 1A

Target: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Andere Bezeichnung: HSPA1A

Hintergrund:

Synonyms: Heat shock 70 kDa protein 1A ,Heat shock 70 kDa protein 1,HSP70-1 ,HSP70.1,HSPA1A,HSPA1, HSX70,

Tissue Specificity: Expressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues. .

Background: HSPA1 (heat shock 70 kDa protein 1A) also known as HSP70-1, HSPA1A, HSP70-1A, HSP72 or HSP70I, is a protein that in humans is encoded by the HSPA1A gene. This intronless gene encodes a 70 kDa heat shock protein which is a member of the heat shock protein 70 family. The HSPA1A gene encodes a predicted 641-amino acid protein. The HSPA1 gene is mapped on 6p21.33. Shimizu et al. (1999) found that peripheral blood mononuclear cells of 18 major depression patients expressed a short HSPA1A transcript that utilized exon 1 rather than exon 2, which is found in the more common HSPA1A transcript. No protein was associated with expression of this short HSPA1A mRNA, possibly due to lack of a TATA box or loss of internal ribosome binding sites. Treatment with BGP-15, a pharmacologic inducer of Hsp72 that can protect against obesity-induced insulin resistance, improved muscular architecture, strength, and contractile function in severely affected diaphragm muscles in mdx dystrophic mice.

Sequence Similarities: Contains 1 HMG box DNA-binding domain.

Molekulargewicht: 70 kDa

Gen-ID: 3303, 3304

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process